Pulsating turbulent flows effects on cleaning in place procedure of straight pipes were investigated for various pulsations parameters (frequency and amplitude) and mean velocities of the flow. Pulsations generation was made with a new system which allows high amplitude of pulsations. Experiments showed the contribution of the different pulsation parameters, in the removal of adhered bacterial spores, in addition to the effect of the mean velocity of the flow. A high level of the cleaning rate is observed despite the reduction of the magnitude of the mean velocity. This result can be explained by the effect of the two pulsations parameters (amplitude and frequency) which ensure a high wall shear rate. The study of the cleaning kinetics has shown the increase of the removal constant rate of spores using pulsed flow in comparison with the use of a steady turbulent flow. (C) 2008 Elsevier Ltd. All rights reserved.