The combination of bio- and chemocatalysts in one-pot is regarded as a breakthrough both for development of new catalytic concepts and for preparation of high-value chemicals. Zeolite nanocrystals (nanozeolites) have been proved as the most used chemical catalysts and as promising supports for enzyme immobilization. These capabilities of nanozeolites endow them with new possibilities for constructing desired platforms for the combination of bio- and chemocatalysis. In this article, several strategies are proposed to develop a one-pot flow microchannel reactor (MCR) by combining enzyme and chemocatalysis via a nanozeolite assembly approach, and the compatibilities of different types of active centers in various assembly strategies are systematically discussed on the basis of the catalytic results of dynamic kinetic resolution (DKR) of sec-alcohol. By precisely controlling the spatial distribution and the ratio of acidic and enzymatic active sites, as well as the reaction conditions, nanozeolite-modified MCR can achieve fast and highly selective one-pot DKR of sec-alcohol within 30 min. (C) 2012 Elsevier Inc. All rights reserved.