A glassy carbon (GC)/graphene/ferritin/glucose oxidase (GOx) anode was developed by using graphene/ferritin biocomposite as an electron transfer enhancer and mediator, respectively. The electrode exhibited good electrocatalytic activity towards the oxidation of glucose. The electrocatalytic oxidation of glucose using GOx modified electrode increased with increasing the concentration of glucose upto 45 mM. The results showed that the graphene/ferritin biocomposite mediator provides enhancement in electron transfer generated at the active cites of GOx to the electrode. All electrochemical measurements were carried out by cyclic voltammetry (CV) and linear sweep voltammetry (LSV). A saturation current density of 66.5 +/- 2 mA cm(-2) at scan rate 100 mV s(-1) for the oxidation of 45 mM glucose was achieved. Copyright (C) 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.