Focusing on the incompatible measurement of the environment property, resources property and economy property, the article aims to make a generalized environment-resource-economy analysis of the processes and to present an overview of different biohydrogen production technologies from the standpoint of the mass production and the whole commercialization chain. One part of the model is the emergy comprehensive efficiency index calculation model, the other is the ternary diagram of structure coefficient for emergy input. The model is used to the organic wastewater of the biohydrogen industrialization demonstration project, and then compared with other biohydrogen and typical renewable energy production technologies. The outputs indicate that the industrialization efficiency of biohydrogen production is available. After the application case of demonstration project, the exploratory work enlightens the similar literature from several aspects. Firstly, the efficiency evaluation model supplies a scientific judgment foundation stone for future laboratory research. Secondly, it provides an alternative theoretical logic to optimize the operation and decision-making of the industrialization and commercialization of the new technologies. Thirdly, it provides a new perspective and quantitative calculation method to effectively integrate that the components comprehensive efficiency of biohydrogen technology change with the different technology processes. Copyright (C) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.