Carbon-supported Pt-Co nanoparticle catalysts (Pt-Co/C) with a mean crystallite size of approximate 2 nm are prepared by a modified NaBH4 reduction method in aqueous solution at room temperature and employed as anode catalyst for direct borohydride-hydrogen peroxide fuel cell (DBHFC). The physical and electrochemical properties are investigated by transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), cyclic voltammetry (CV), chronoamperometry (CA) and fuel cell test. The results show that Pt-Co/C catalyst presents more excellent performance as DBHFC anode catalyst compared with Pt/C catalyst, especially, Pt67Co33/C catalyst presents the highest catalytic activity for BH4- electrooxidation among all as-prepared catalysts. The single DBHFC using Pt67Co33/C as anode catalyst and Pt/C as cathode catalyst obtains the maximum power density as high as 79.7 mW cm(-2) at 25 degrees C. Copyright (C) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.