In this paper, experimental and numerical investigations of the effects of poly-benzimidazole (PBI) loading and operating temperature on a high-temperature proton exchange membrane fuel cell (PEMFC) performance are carried out. Experiments related to a PBI-based PEMFC are performed and a two-dimensional (2-D) simulation model is developed to numerically predict the cell characteristics. Variations of 5-30 wt% in PBI amount in the catalyst layer (CL) and 160-200 degrees C in cell temperature are considered. On the basis of the experimental and numerical results, the negative effect of PBI content and positive effect of operating temperature on the cell performance can be precisely captured. These effects can also be shown by measurements of the impedance spectrum and predictions of O-2 concentration and current density distributions. In addition, non-uniform distributions in the O-2 concentration and the current density in the cathode compartment are also shown in the model simulation results. Cell performance curves predicted by the present model correspond well with those obtained from experimental measurements, showing the applicability of this model in a PBI-based PEMFC. Copyright (C) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.