The number of energy-consuming products is increasing. The use of renewable energy has already become the global goal to solve the global energy shortage. The development of the fuel cell is an important subject. This study plots the V-I characteristic curve of fuel cells using LabView. The experimental result demonstrates that the efficiency with which a fuel cell generates electricity when controlled by a Fuzzy controller is 37% - which significantly exceeds that obtained using a PID controller, 14.67%. Hence, much less bio-hydrogen is wasted, so the cost of generating electricity using the fuel cell is lower. Moreover, the Fuzzy controller need not be used for a long time as a PID controller to estimate and test variables. This research will contribute to the development of the bio-hydrogen fuel cell generators. Crown Copyright (C) 2008 Published by Elsevier Ltd on behalf of International Association for Hydrogen Energy. All rights reserved.