천연고분자 분해성 필름의 생체적합성 연구

황성규; 이기창; 임국환

Journal of the Korean Industrial and Engineering Chemistry, Vol.10, No.6, 939-943, October, 1999

Export Citation

천연고분자 분해성 필름의 생체적합성 연구

Biocompatibility of Biodegradable Films by Natural Polymers

황성규, 이기창, 임국환

초록

최근에 생분해성 고분자를 이용한 의료용재료로의 응용에 대한 관심이 고조되고 있다. 본 연구에서는 천연고분자(xanthan, locust bean, guar gum, chitosan 및 algin)를 생체재료로 사용할 목적으로 용액주조법에 의하여 분해성 필름을 제조하였다. 천연고분자로부터 제조한 생체재료의 가능성을 피부이식제로서 생체적합성 측정에 의하여 평가하였다. 이들을 실험동물의 등부위에 피하 삽입하여 필름의 시간에 따른 구조적, 중량적 변화와 랫드의 혈액학적 변화를 측정하여 생체적합성을 연구하였다. 랫드에 대한 테스트결과 locust bean 과 guar gum은 삽입 24시간, 48시간 후 조직에서의 생체 이물 반응에 의한 생체 부적합성을 나타내었으며, 일부 천연고분자로부터 제조한 분해성 필름은 단기간의 이상적인 생체재료로서의 가능성을 나타내었다.

Recently there has been an explosion of interest in the topic of biodegradable polymers for medical applications. In this study, films were prepared by solution casting method using natural polymers (Xanthan, locust bean, guar gum, chitosan and algin) as biomaterials. Biocompatibility of films prepared from natural polymer as a skin implant was evaluated. These biodegradable films were subcutaneously implanted in the back of rats and their biodegradability was investigated by the evaluation of changes in structure, film weight and hematology as a function of time for the biotransformation. The result of rats test showed that locust bean and guar gum induced some suspects of non-biocompatibility in the tissue by foreign body reaction 24 and 48 hrs after implantation. These results showed the potential of partial biodegradable films prepared from natural polymer for ideal skin biomaterials at short period.

Keywords:biocompatibility;biomaterial;natural polymer;biotransformation

[References]

Langer RS, Peppas NA, Biomaterials, 2, 201 (1981)
Vert M, Christel P, Leray J, "Macromolecular Biomaterials," CRC Press, Boca Raton, 120 (1984)
Lee HB, "Frontiers of Macromolecular Science," edited by T. Saegusa, T. Higashimura, and A. Abe, Blackwell Scientific Publications, London, 579 (1989)
Williams DF, "Biocompatibility of Clinical Implant Materials," vol. II, CRC Press, Boca Raton, Florida, U.S.A., 249 (1981)
Lee HB, Kim SS, Khaug G, "Polymeric Biomaterials," Biomedical Engineering Handbook, Section IV, Ch. 42, J.D. Bronzino Ed., CRC Press, Boca Raton, 211 (1995)
Zhao Q, Marchant RE, Hiltner A, Polymer, 28, 2040 (1987)
Barenberg SA, Chairperson P, J. Biomed. Mater. Res., 22, 1267 (1988)
Heller J, Baker RW, "Theory and Practice of Controlled Drug Delivery from Bioerodible Polymer," In Controlled Release of Bioactive Materials, Academic Press, New York, 1 (1980)
Ziats NP, Miller KM, Anderson M, Biomaterials, 9, 5 (1998)
Allison AC, Byars NE, Mol. Immunol., 28, 279 (1991)
Boretos JW, "In Biocompatibility of Clinical Implant Materials," Vol. II, Williams D.F.(Ed.), CRC Press, Boca Raton, 127 (1981)
Park JB, Lakes R, ""Biomaterials," 2nd Ed., Plenum Press, New York, 1 (1992)
Shiroshu T, Toyama Y, "New Toxicological Test Guideline," Realize Inc., Tokyo, 12 (1988)
Chu CC, J. Biomed. Mater. Res., 15, 19 (1981)
Mitruka BM, Rawnsley HM, "Clinical Biochemical and Hematological Reference Values in Normal Experimental Animals and Normal Humans," 2nd Ed., Masson Publishing, New York, 58 (1981)
Baker HJ, Lindsey JR, Weisbroth SH, "The Laboratory Rat," Academic Press, New York, 21 (1979)
Rol F, Industrial Gums, R.L. Whistler Ed., Academic Press, 2nd Ed., New York, 323 (1973)
Jain NC, "Schalm's Veterinary Hematology," 4th Ed, Lea & Febiger, Philadelphia, 676 (1986)
Murphy P, "The Neutrophil," Plenum, New York, 26 (1976)

[Referenced By]

[1] Langer RS, Peppas NA, Biomaterials, 2, 201 (1981)

[2] Vert M, Christel P, Leray J, "Macromolecular Biomaterials," CRC Press, Boca Raton, 120 (1984)

[3] Lee HB, "Frontiers of Macromolecular Science," edited by T. Saegusa, T. Higashimura, and A. Abe, Blackwell Scientific Publications, London, 579 (1989)

[4] Williams DF, "Biocompatibility of Clinical Implant Materials," vol. II, CRC Press, Boca Raton, Florida, U.S.A., 249 (1981)

[5] Lee HB, Kim SS, Khaug G, "Polymeric Biomaterials," Biomedical Engineering Handbook, Section IV, Ch. 42, J.D. Bronzino Ed., CRC Press, Boca Raton, 211 (1995)

[6] Zhao Q, Marchant RE, Hiltner A, Polymer, 28, 2040 (1987)

[7] Barenberg SA, Chairperson P, J. Biomed. Mater. Res., 22, 1267 (1988)

[8] Heller J, Baker RW, "Theory and Practice of Controlled Drug Delivery from Bioerodible Polymer," In Controlled Release of Bioactive Materials, Academic Press, New York, 1 (1980)

[9] Ziats NP, Miller KM, Anderson M, Biomaterials, 9, 5 (1998)

[10] Allison AC, Byars NE, Mol. Immunol., 28, 279 (1991)

[11] Boretos JW, "In Biocompatibility of Clinical Implant Materials," Vol. II, Williams D.F.(Ed.), CRC Press, Boca Raton, 127 (1981)

[12] Park JB, Lakes R, ""Biomaterials," 2nd Ed., Plenum Press, New York, 1 (1992)

[13] Shiroshu T, Toyama Y, "New Toxicological Test Guideline," Realize Inc., Tokyo, 12 (1988)

[14] Chu CC, J. Biomed. Mater. Res., 15, 19 (1981)

[15] Mitruka BM, Rawnsley HM, "Clinical Biochemical and Hematological Reference Values in Normal Experimental Animals and Normal Humans," 2nd Ed., Masson Publishing, New York, 58 (1981)

[16] Baker HJ, Lindsey JR, Weisbroth SH, "The Laboratory Rat," Academic Press, New York, 21 (1979)

[17] Rol F, Industrial Gums, R.L. Whistler Ed., Academic Press, 2nd Ed., New York, 323 (1973)

[18] Jain NC, "Schalm's Veterinary Hematology," 4th Ed, Lea & Febiger, Philadelphia, 676 (1986)

[19] Murphy P, "The Neutrophil," Plenum, New York, 26 (1976)