미생물 고정화를 위한 광경화성 하이드로겔의 합성과 특성

김조웅; 이정복; 김두현; 황정민; 조종수; 최영훈; 정대원

Journal of the Korean Industrial and Engineering Chemistry, Vol.10, No.6, 852-856, October, 1999

Export Citation

미생물 고정화를 위한 광경화성 하이드로겔의 합성과 특성

Synthesis and Characteristics of Photo-crosslinkable Hydrogel for Microbial Immobilization

김조웅, 이정복, 김두현, 황정민, 조종수, 최영훈, 정대원

초록

산업페수중의 질소·인 제거에 유용한 미생물을 고정화할 수 있는 담체의 제조를 목적으로 하여, 양 말단이 methacryl기로 치환된 poly(ethyleneglycol)(PEG) 및 Poly(tetramethylene glycol)(PTMG)을 합성하였다. 합성된 프레폴리머와 UV조사하에서 가교한 하이드로겔의 구조는 ¹H-NMR, FT-IR 분광기로 확인하였다. 프레폴리머 사슬의 길이(M.W. 1,000∼8,000) 또는 PEG/PTMG의 혼합비를 달리하면서 광경화된 하이드로겔을 제조하였다. 광경화물의 친수성, 함수율, 기계적 강도 등과 같은 광경화 특성 및 pore size 등을 조사한 결과, 분자량 1000인 PEG 및 PEG(MW1000)와 PTMG (MW2900)를 7:3의 비율로 제조한 하이드로겔에서 최적의 결과를 나타내었다.

The objective of theis study was to prepare hydrogel beads which were useful microbial immobilization to remove nitrogen and phosphorous in the industrial wastewater. Two different polyols(PEG, PTMG) terminated with photo-crosslinkable methacrylate groups were synthesized. Structures of the prepolymers and the UV cured hydrogels were characterized by using ¹H-NMR and FT-IR spectroscopy. Water content, mechanical strength and pore sizes of the hydrogels having different MW of polyols and different ratios of PEG/PTMG were investigated. Hydrogels prepared from PEG(MW1000) only or the mixture of PEG(MW1000) and PTMG(MW2900) with 7:3 by weight were considered as potential candidates for the matrix for the immobilization of microorganism.

Keywords:immobilization;UV cured hydrogels;polyols;microorganism

[References]

Zeng L, Stejskal EO, Appl. Spectrosc., 50, 1402 (1996)
Jen AC, Wake MC, Mikos AG, Biotechnol. Bioeng., 50(4), 357 (1996)
Lee YM, Kim SS, Polymer, 38(10), 2415 (1997)
Mathur AM, Moorjani SK, Scranton AB, Macromol. Chem. Phys., C36(2), 405 (1996)
Vyavahare N, Kohn J, J. Polym. Sci. A: Polym. Chem., 32(7), 1271 (1994)
King G, Goosen MFA, "Fundamentals of Animal Cell Encapsulation and Immobilization," pp. 1-6, CRC Press (1993)
Goosen MFA, "Fundamentals of Animal Cell Encapsulation and Immobilization," pp. 43-54, CRC Press (1993)
Fouassier JP, Rabek JF, "Radiation Curing in Polymer Science and Technology," Elsevier Sci., NY (1993)
Kim BK, Lee KH, Kim HD, J. Appl. Polym. Sci., 60(6), 799 (1996)
Kim HD, Kang SG, Ha CS, J. Appl. Polym. Sci., 46, 1339 (1992)
Chiou BS, English RJ, Khan SA, Macromolecules, 29(16), 5368 (1996)
Priola A, Gozzelino G, Ferrero F, Malucelli G, Polymer, 34, 3653 (1993)
順藤隆一, "微生物固定化法によるく排水處理," 産業用水調査會, 日本 (1987)
Ichimura K, Mishima K, Watanabe A, J. Water Waste, 29, 742 (1987)
Iida T, Sakamoto M, Izumida H, Akagi T, J. Ferment. Bioeng., 75, 32 (1993)
大律降行, 木下雅設, "高分子合成の實驗法," 化學同人, 日本 (1976)
Du YG, Tyagi RD, Bhamidimarri R, Process Biochem., 31(8), 753 (1996)

[1] Zeng L, Stejskal EO, Appl. Spectrosc., 50, 1402 (1996)

[2] Jen AC, Wake MC, Mikos AG, Biotechnol. Bioeng., 50(4), 357 (1996)

[3] Lee YM, Kim SS, Polymer, 38(10), 2415 (1997)

[4] Mathur AM, Moorjani SK, Scranton AB, Macromol. Chem. Phys., C36(2), 405 (1996)

[5] Vyavahare N, Kohn J, J. Polym. Sci. A: Polym. Chem., 32(7), 1271 (1994)

[6] King G, Goosen MFA, "Fundamentals of Animal Cell Encapsulation and Immobilization," pp. 1-6, CRC Press (1993)

[7] Goosen MFA, "Fundamentals of Animal Cell Encapsulation and Immobilization," pp. 43-54, CRC Press (1993)

[8] Fouassier JP, Rabek JF, "Radiation Curing in Polymer Science and Technology," Elsevier Sci., NY (1993)

[9] Kim BK, Lee KH, Kim HD, J. Appl. Polym. Sci., 60(6), 799 (1996)

[10] Kim HD, Kang SG, Ha CS, J. Appl. Polym. Sci., 46, 1339 (1992)

[11] Chiou BS, English RJ, Khan SA, Macromolecules, 29(16), 5368 (1996)

[12] Priola A, Gozzelino G, Ferrero F, Malucelli G, Polymer, 34, 3653 (1993)

[13] 順藤隆一, "微生物固定化法によるく排水處理," 産業用水調査會, 日本 (1987)

[14] Ichimura K, Mishima K, Watanabe A, J. Water Waste, 29, 742 (1987)

[15] Iida T, Sakamoto M, Izumida H, Akagi T, J. Ferment. Bioeng., 75, 32 (1993)

[16] 大律降行, 木下雅設, "高分子合成の實驗法," 化學同人, 日本 (1976)

[17] Du YG, Tyagi RD, Bhamidimarri R, Process Biochem., 31(8), 753 (1996)