Special rose-like copper (I) sulfide nanostructure (Cu2S NRS) made up of thickness nanoplates were successfully synthesized on copper rod by a simple method. Then the as-prepared Cu2S NRS/Cu rod can be used as integrated electrode (called as Cu2S NRS/CRIE) to nonenzymatic detect the cholesterol (CT). The electrochemical properties show these integrated electrode revealed wide linear range from 0.01 mM to 6.8 mM and low detection limit of 0.1 mu M (S/N =3) for the detection of cholesterol. What's more, the proposed sensor displays excellent selectivity, good stability, and satisfying repeatability. (C) 2014 Elsevier Ltd. All rights reserved.