We report controllable fabrication and photoconductive properties of free-standing crystallized TiO2 nanotube (TNT) membranes. The TNT membranes are synthesized by a two-step anodic procedure, in which dependence of the TNT morphology and detachment on the applied voltage is experimentally present. Due to the large surface area-to-volume ratio and high quality crystal structure, the membrane exhibits a sensitive spectral response to the ultra violet (UV) light. The spectral response at 320 nm is similar to 30A/W under 5.0V bias, corresponding to external quantum efficiency of 10,695%. The photoresponse origins from internal gain induced by desorption of oxygen molecules from the nanotube surfaces and reduction of the barrier at neighboring tubes under UV illumination. This work extracts intrinsic photoconductive properties of TNT membranes and is beneficial to the future design of optoelectronic devices. (C) 2013 Elsevier Ltd. All rights reserved.