In the present study the electrocatalytic behavior of platinum nanoparticle-decorated multi-walled carbon nanotube clusters vertically aligned on a screen-printed gold nanofilm electrode substrate (SPGFE/MWCNTC/PtNP) toward hydrogen peroxide was intensively investigated. Oriented multi-walled carbon nanotube clusters (MWCNTC) were firstly well-organized onto the screen-printed gold film electrode (SPGFE) surface by covalent immobilization, and then platinum nanoparticles (PtNPs) dispersed on the MWCNTC structure were in situ synthesized by electrochemically reducing chloroplatinic acid. It was demonstrated that the MWCNTC/PtNP hybrid structure on the SPGFE substrate could significantly enhance the electrochemical reduction of hydrogen peroxide in neutral solutions. Compared with platinum nanoparticles loading on disordered multi-walled carbon nanotubes (SPGFE/MWCNT/PtNP), the proposed SPGFE/MWCNTC/PtNP exhibited more sensitive responses for hydrogen peroxide reduction. The fabricated electrode provided linear current responses for hydrogen peroxide in the concentration range from 5 to 2000 mu M with a detection limit of 1.23 mu M. The non-enzymatic sensor also offered good reproducibility and high selectivity for hydrogen peroxide detection. The carbon nanotube cluster/platinum nanoparticle hybrid system holds great promise as a scaffold to fabricate enzyme biosensors. (C) 2012 Elsevier Ltd. All rights reserved.