In this paper, monodisperse polydopamine (PDA)-Ag hybrid hollow microspheres were synthesized by self-polymerization of dopamine on ZnO microspheres as sacrificial templates. The templates were dissolved in basic media and in situ reduced the incorporated Ag+ ion with ascorbic acid. The as-prepared PDA-Ag composites modified glassy carbon electrode exhibited a broad linear range of 0.092-20 mM for H2O2 determination, with a remarkable sensitivity of 6.79 mu A mM(-1), a detection limit of 1.97 mu M (S/N = 3), and rapid response (within 5 s). Moreover, the electrode showed good reproducibility, anti-interferant ability and long-term stability. (C) 2011 Elsevier Ltd. All rights reserved.