A novel kind of nanofiltration (NF) hollow fiber membranes with high density of negative charges and relative high permeate flux was prepared by grafting of 2-acrylamido-2-methylpropanesulfonic add (AMPS) onto a polysulfone (PSf) ultrafiltration hollow fiber membrane using the simultaneous electron beam radiation-induced method. ATR-FTIR and FESEM analyses revealed that the monomer was grafted on both the outer and inner surfaces of the membrane. The effects of irradiation dose and monomer concentration on the mean pore size and performance of the membranes were investigated. The prepared NF membranes were used for removal of Cr(VI) from aqueous solution under varying conditions such as pH value, initial Cr(VI) concentration and coexisting ions. The experimental results showed that Cr(VI) could be effectively removed by the resulting NF membranes when the pH of the solution was higher than 8 and the performance of Cr(VI) removal was nearly not affected by the presence of Na2SO4 and NaCl in the experimental range. It was indicated that the high negative charge density resulting from the high degree of grafting made the grafted membranes achieve nanofiltration performance at a relative large pore size. (C) 2014 Elsevier B.V. All rights reserved.