The photocatalytic inactivation of two different wastewater bacteria, Gram-positive (+ve) bacterium Microbacterium barkeri and Gram-negative (ye) bacterium Escherichia coil, were comparably investigated with natural sphalerite (NS) as a novel low-cost photocatalyst. The natural sphalerite was able to inactivate 10(5) cfu/mL of the Gram-positive (+ve) bacterium M. barkeri within 10 h at neutral pH while 10(7) cfu/mL of the Gram-negative (ve) bacterium E. coil was inactivated within 6 h. In addition, the pH effect on the photocatalytic inactivation of bacteria was studied, and the results suggested that the amount of H2O2 produced under different pH influenced the bacterial inactivation efficiency. The major reactive species for photocatalytic inactivation by the NS were determined using multiple scavengers, and H2O2 was found to be the major ROS in M. barkeri inactivation, while both H2O2 and e(-) contributed to E. coli inactivation. Different mechanism between Gram-positive (+ve) and Gram-negative (-ve) bacteria was mainly due to the different thickness of the cell wall, as indicated by direct observation of the cell wall and cell membrane by transmission electron microscopy and leakage detection of potassium ions. (C) 2013 Elsevier B.V. All rights reserved.