Lipase from Burkholderia cepacia was immobilized on modified attapulgite by cross-linking reaction for biodiesel production with jatropha oil as feedstock. Effects of various factors on biodiesel production were studied by single-factor experiment. Results indicated that the best conditions for biodiesel preparation were: 10 g jatropha oil, 2.4 g methanol (molar ratio of oil to methanol is 1:6.6) being added at 3 h intervals, 7 wt% water, 10 wt% immobilized lipase, temperature 35 degrees C, and time 24 h. Under these conditions, the maximum biodiesel yield reached 94%. The immobilized lipase retained 95% of its relative activity during the ten repeated batch reactions. The half-life time of the immobilized lipase is 731 h. Kinetics was studied and the V,a of the immobilized lipases were 6.823 mmol L-1. This immobilized lipase catalyzed process has potential industrial use for biodiesel production to replace chemical-catalyzed method. (C) 2013 Elsevier Ltd. All rights reserved.