In this study, performance of laboratory-scale membrane bioreactor (MBR) was evaluated in treating high strength domestic wastewater under two nitrogen loading rates (NLR) i.e., 0.15 and 030 kg/m(3)/d in condition 1 and 2, respectively, while organic loading rate (OLR) was constant at 3 kg/m(3)/d in both conditions. Removal efficiencies of COD were above 95.0% under both NLR conditions. Average removal efficiencies of ammonium nitrogen (NH4+-N), total nitrogen (TN) and total phosphorus (TP) were found to be higher in condition 1 (90.5%, 74.0%, and 38.0%, respectively) as compared to that in Condition 2 (89.3%, 35.0%, and 14.0%, respectively). With increasing NLR, particle size distribution shifted from narrow (67-133 mu m) towards broader distribution (3-300 mu m) inferring lower cake layer porosity over membrane fibers. Soluble extracellular polymer substance (sEPS) concentration increased at higher NLR due to biopolymers released from broken flocs. Higher cake layer resistance (R-c) contributed towards shorter filtration runs during condition 2. (c) 2013 Elsevier Ltd. All rights reserved.