The enzymatic hydrolysate of sweetpotato vines (SVH) characterized as an effective nutrients supplier with low nitrogen availability was firstly used as a substrate by Trichosporon fermentans for single cell oils (SCOs) production. Batch-fermentation experiments on various SVH based media suggested that co-fermentation of SVH and some high-sugar content substrates would be much more efficient and less-cost for SCOs production. A lipid yield of 9.6 gl(-1) with a lipid content of 35.6% was achieved on the SVH without any addition, while 27.6 and 17.7 gl(-1) lipid were respectively obtained on the fructose supplemented SVH media and the SVH mixed with acid treated wheat straw hydrolysate (WSH). The positive effect of SVH on the lipid production of T. fermentans was further demonstrated with a kinetic investigation revealing that SVH had a remarkable promoting effect on the biomass formation and the substrate uptake. (C) 2012 Elsevier Ltd. All rights reserved.