Two anoxic/oxic sequencing batch reactors (A/O SBRs) were operated to investigate the effects of step-feed on granulation processes and performances of partial nitrifying granules (PNG). R1 was operated in a traditional single-feed mode, while a two-step-feed strategy was used in R2. Results showed that R1 had a faster granulation process and better performance in maintaining partial nitrification compared with R2, indicating that the step-feed mode had a negative effect of on formation of PNG. However, after full granulation, PNG in both reactors had similar properties in terms of suspended solids (MLSS), sludge volume index (SVI) and granule size. Moreover, mature granules in R2 had a higher nitrite accumulation rate than that in R1. Step-feed strategy was also observed to enhance denitrification and TN removal, as well as ammonia oxidation. It can be concluded that step-feed was unfavorable for cultivating PNG, but it significantly improved the nitrogen removal performance of PNG. (C) 2012 Elsevier Ltd. All rights reserved.