Uncertainty exists over the magnitude of greenhouse gas (GHG) emissions associated with open land conversion to short-rotation woody biomass crops (SRWC) for bioenergy in the Northern U.S. Lake States. GHG debts incurred at the plantation establishment phase may delay the climate mitigation benefits of SRWC production. To better understand GFIG debts associated with converting open lands to SRWC, we established research plantations with willow (Salix spp), hybrid-poplar (Populus spp.), and control plots in spring 2010 at two sites in northern Michigan (ES) and Wisconsin (RH). These sites had similar climates, but differed in time since last cultivation: 5 vs. 42 years. To address the short-term effects of plantation establishment, we compared two-year biomass production and GHG emissions. We hypothesized that the long-idle ES site, with higher initial soil C and N stocks, would have higher GHG emissions following conversion compared to the recently-idle RH site, but that this would be balanced in part by greater SRWC productivity at the ES site. As hypothesized, grassland conversion resulted in two-year net GHG emissions due to land conversion of 43.21 and 33.02 Mg-CO(2)eq ha(-1) for poplar and willow at ES that was far greater than the 4.81 and 1.54 Mg-CO(2)eq ha(-1) for poplar and willow at RH. Contrary to our hypothesis, we did not observe greater SRWC productivity at ES, which will take longer than RH to reach C neutrality and begin mitigating GHG emissions. Our results show that site-specific soil and management factors determine the magnitude of GHG emissions. Published by Elsevier Ltd.