This paper aims to present the energy balance of biodiesel production from palm oil in the Amazon. The Life Cycle Assessment (LCA) is made for the three phases of the production of biodiesel from palm: agriculture, oil production and biodiesel production. For each phase is considered the energy inputs and materials, and energy production of the product and co-products. The analyzed data on agricultural production and oil were collected in two Amazon companies DENTAUA and AGROPALMA. Regarding biodiesel production data concerning the amount of oil, ethanol, biodiesel, glycerol were obtained from the transesterification reaction considering 98% yield. The results show the energetic costs at each stage of biodiesel production. (C) 2011 Published by Elsevier Ltd.