Sequence analysis and heterologous expression of the wool cuticle-degrading enzyme encoding genes in Fusarium oxysporum 26-1

Chaya E; Suzuki T; Karita S; Hanya A; Yoshino-Yasuda S; Kitamoto N

Journal of Bioscience and Bioengineering, Vol.117, No.6, 711-714, 2014

DOI10.1016/j.jbiosc.2013.11.012 Export Citation

Sequence analysis and heterologous expression of the wool cuticle-degrading enzyme encoding genes in Fusarium oxysporum 26-1

Chaya E, Suzuki T, Karita S, Hanya A, Yoshino-Yasuda S, Kitamoto N

Two protease-like proteins, KrtA and KrtC, were identified in Fusarium oxysporum 26-1. Genes coding these proteins, krtA and krtC, were isolated and characterized. Recombinant KrtA (rKrtA) and KrtC (rKrtC) were successfully expressed in Aspergillus oryzae and secreted. The combination of rKrtA and rKrtC completely removed the cuticle of wool fibers. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.

Keywords:Fusarium oxysporum;Wool cuticle;Protease;Heterologous expression;Aspergillus oryzae