Steam reforming of bio-oil was an available low carbon technology to produce biomass-syngas. In this article, a series of catalysts have been prepared; the activity of catalysts was examined in a fluidized bed reactor. In these tests, carbon conversion, the mole fraction of H-2, the mole of CO, and the ratio of H-2/CO were taken as an index of the catalyst activity and the nickel/modified dolomite catalyst was chosen as the best catalyst for the bio-oil steam reforming to prepare biomass-syngas. The effects of reaction conditions, such as reaction temperature, steam-to-carbon mole ratio (S/C), and weight hourly space velocity (WHSV), on catalytic reforming were studied and the results indicated that the optimum conditions were obtained at 700 degrees C, S/C was 14, and WHSV was 0.5 h(-1). The mole ratio of H-2/CO was more than 2 and carbon conversion was near 80% at the optimum conditions.