Electric drive vehicle technologies are being considered as possible solutions to mitigate environmental problems and fossil fuels dependence. Several studies have used life cycle analysis technique, to assess energy use and CO2 emissions, addressing fuels Well-to-Wheel life cycle or vehicle's materials Cradle-to-Grave. However, none has considered the required infrastructures for fuel supply. This study presents a methodology to evaluate energy use and CO2 emissions from construction, maintenance and decommissioning of support infrastructures for electricity and fossil fuel supply of vehicles applied to Portugal case study. Using Global Warming Potential and Cumulative Energy Demand, three light-duty vehicle technologies were considered: Gasoline, Diesel and Electric. For fossil fuels, the extraction well, platform, refinery and refuelling stations were considered. For the Electric Vehicle, the Portuguese 2010 electric mix, grid and the foreseen charging point's network were studied. Obtained values were 0.6-1.5 gCO(2eq)/km and 0.03-0.07 MJ(eq)/km for gasoline, 0.6-1.6 gCO(2eq)/km and 0.02-0.06 MJ(eq)/km for diesel, 3.7-8.5 gCO(2eq)/km and 0.06-0.17 MJ(eq)/km for EV. Monte Carlo technique was used for uncertainty analysis. We concluded that EV supply infrastructures are more carbon and energetic intensive. Contribution in overall vehicle LCA does not exceed 8%. (C) 2011 Elsevier Ltd. All rights reserved.