Zwitterionic fluorescent polymers (ZFPs) are developed by using poly(sulfobetaine methacrylate), NIPAAm, and BODIPY fluorophores to examine the patterning characteristics based on mussel-inspired surface modification. Water contact angle measurements of ZFP show changes in the angle upon altering the pH. The ZFP was developed on PDMS stamps and imprinted onto a glass substrate, which provided a green fluorescent micropattern under a fluorescence microscope, making the ZFP a powerful tool for development of a useful patterned substrate for various applications.