Wheat bran is a vegetal fibrous material rich in different chemicals (e.g. arabinoxylan) that can be used as food ingredient. To increase the extractability of these compounds, production of small fiber particles and therefore milling for long periods of time have been proposed. Different laboratory- and pilot-scale technologies for fine and ultrafine grinding of wheat bran were evaluated in this publication. Various milling tools were applied both under wet and dry conditions. Generally better results were obtained when grinding the wheat bran under dry condition. Optimum results were achieved by a combination of ball milling followed by treatment of the powder with a jet air mill. Electron microscopic evaluation confirmed the sub-micrometer size of most particles and the presence of agglomerates.