Biochemical and Biophysical Research Communications, Vol.445, No.4, 791-794, 2014
Efficient generation of genome-modified mice via offset-nicking by CRISPR/Cas system
The mammalian zygote-mediated CRISPR/Cas system can efficiently generate targeted genome-modified animals. However, this system is limited by the risk of off-target mutations. Here we show that offset-nicking by Cas9 nickase and paired gRNAs allows us to generate region deleted mice and targeted knock-in mice without off-target mutations. (c) 2014 The Authors. Published by Elsevier Inc.