Thickness, refractive index and composition of coatings consisting of polymer brushes of poly(N-isopropyl acrylamide), fabricated with different polymerization times, and oligoperoxide, grafted to the glass surface with (3-aminopropyl) triethoxysilane, were determined using ellipsometry and AFM measurements at all the formation stages. Optimum grafting conditions were determined for each component of the composite film. Dry film measurements and in situ temperature depending swelling experiments were performed by multiple angle of incidence (MAI) ellipsometry. The temperature phase transition in water solution induced by collapse of the polymer brushes was detected using ellipsometry. The ability of coatings consisting of complex polymer brushes to modify physicochemical interfacial properties depending on temperature is demonstrated. (C) 2013 Elsevier B.V. All rights reserved.