Effect of shear stress on the growth of continuous culture of Synechocystis PCC 6803 in a flat-panel photobioreactor

Min-Gyu Sung; Won-Sub Shin; Woong Kim; Jong-Hee Kwon; Ji-Won Yang

Korean Journal of Chemical Engineering, Vol.31, No.7, 1233-1236, July, 2014

DOI10.1007/s11814-014-0029-z Export Citation

Effect of shear stress on the growth of continuous culture of Synechocystis PCC 6803 in a flat-panel photobioreactor

Min-Gyu Sung¹, Won-Sub Shin¹, Woong Kim¹, Jong-Hee Kwon^{1, 2, †}, and Ji-Won Yang^{1, 3, †}

¹Department of Chemical and Biomolecular Engineering, KAIST, Yuseong-gu, Daejeon 305-701, Korea
²Plant Biochemistry, Faculty of Biology & Biotechnology, Ruhr University Bochum, D-44780 Bochum, Germany
³Advanced Biomass R&D Center, KAIST, Yuseong-gu, Daejeon 305-701, Korea

E-mail:,

The effect of hydrodynamic forces generated by air bubbles on cell growth of continuous culture of Synechocystis PCC 6803 was studied in a flat-panel photobioreactor. Keeping all relevant parameters constant enables the optimization of individual parameters, for which a continuous cultivation approach has significant advantages. Continuous culture of Synechocystis PCC 6803 was cultivated under different gas velocities from 0.022 m s^(-1) up to 0.128 m s^(-1). Based on direct determination of effective growth rate at constant cell densities, cell damage due to shear stress induced by the increasing gas velocity at the sparger was directly observed. A significant decrease of effective growth rate was observed at gas velocity of 0.085 m s^(-1) generated at the gas flow rate of 200 ml min^(-1), indicating cell damage by shear stress. Optimization of gas volume and the development of an effective aeration system corresponding to a given reactor setup is important to realize a reliable cell growth.

Keywords:Shear Stress;Bioprocess Optimization;Gas Flow Rate;Continuous Cultivation;Photobioreactor

[References]

Allakhverdiev SI, Thavasi V, Kreslavski VD, Zharmukhamedov SK, Klimov VV, Ramakrishna S, Los DA, Mimuro M, Nishihara H, Carpentier R, J. Photochem. Photobiol. C: Photochemistry Reviews, 11, 101 (2010)
Dutta D, De D, Chaudhuri S, Bhattacharya SK, Microb. Cell Fact., 4, 36 (2005)
Esper B, Badura A, Rogner M, Trends in Plant Science, 11, 543 (2006)
Vijayendran B, Journal of Business Chemistry, 7, 109 (2010)
Ragauskas AJ, Williams CK, Davison BH, Britovsek G, Cairney J, Eckert CA, Frederick WJ, Hallett JP, Leak DJ, Liotta CL, Science, 311, 484 (2006)
Demirbas A, Prog. Energy Combust. Sci., 33, 1 (2007)
Chisti Y, Biotechnol. Adv., 25, 294 (2007)
Noue J, Laliberte G, Proulx D, J. Appl. Phycol., 4, 247 (1992)
Janssen M, Tramper J, Mur LR, Wijffels RH, Biotechnol. Bioeng., 81(2), 193 (2003)
Posten C, Engineering in Life Sciences, 9, 165 (2009)
Barbosa MJ, Albrecht M, Wijffels RH, Biotechnol. Bioeng., 83(1), 112 (2003)
Contreras A, Garcia F, Molina E, Merchuk J, Biotechnol. Bioeng., 60, 317 (2000)
Kwon JH, Rogner M, Rexroth S, J. Biotechnol., 162(1), 156 (2012)
Oie S, Obayashi A, Yamasaki H, Furukawa H, Kenri T, Takahashi M, Kawamoto K, Makino S, Biological and Pharmaceutical Bulletin, 34, 1325 (2011)
Czitrom V, American Statistician, 126 (1999)
Tang HY, Chen M, Ng KYS, Salley SO, Biotechnol. Bioeng., 109(10), 2468 (2012)
Havlik I, Lindner P, Scheper T, Reardon KF, Trends in Biotechnology, 31(7), 406 (2013)
Shcolnick S, Shaked Y, Keren N, Biochim. Biophys. Acta (BBA)-Bioenergetics, 1767, 814 (2007)
Zhang X, Liu S, Chen X, Ener. Procedia, 11, 2121 (2011)
Liberton M, Berg RH, Heuser J, Roth R, Pakrasi HB, Protoplasma, 227, 129 (2006)

[Related Articles]

[1] Allakhverdiev SI, Thavasi V, Kreslavski VD, Zharmukhamedov SK, Klimov VV, Ramakrishna S, Los DA, Mimuro M, Nishihara H, Carpentier R, J. Photochem. Photobiol. C: Photochemistry Reviews, 11, 101 (2010)

[2] Dutta D, De D, Chaudhuri S, Bhattacharya SK, Microb. Cell Fact., 4, 36 (2005)

[3] Esper B, Badura A, Rogner M, Trends in Plant Science, 11, 543 (2006)

[4] Vijayendran B, Journal of Business Chemistry, 7, 109 (2010)

[5] Ragauskas AJ, Williams CK, Davison BH, Britovsek G, Cairney J, Eckert CA, Frederick WJ, Hallett JP, Leak DJ, Liotta CL, Science, 311, 484 (2006)

[6] Demirbas A, Prog. Energy Combust. Sci., 33, 1 (2007)

[7] Chisti Y, Biotechnol. Adv., 25, 294 (2007)

[8] Noue J, Laliberte G, Proulx D, J. Appl. Phycol., 4, 247 (1992)

[9] Janssen M, Tramper J, Mur LR, Wijffels RH, Biotechnol. Bioeng., 81(2), 193 (2003)

[10] Posten C, Engineering in Life Sciences, 9, 165 (2009)

[11] Barbosa MJ, Albrecht M, Wijffels RH, Biotechnol. Bioeng., 83(1), 112 (2003)

[12] Contreras A, Garcia F, Molina E, Merchuk J, Biotechnol. Bioeng., 60, 317 (2000)

[13] Kwon JH, Rogner M, Rexroth S, J. Biotechnol., 162(1), 156 (2012)

[14] Oie S, Obayashi A, Yamasaki H, Furukawa H, Kenri T, Takahashi M, Kawamoto K, Makino S, Biological and Pharmaceutical Bulletin, 34, 1325 (2011)

[15] Czitrom V, American Statistician, 126 (1999)

[16] Tang HY, Chen M, Ng KYS, Salley SO, Biotechnol. Bioeng., 109(10), 2468 (2012)

[17] Havlik I, Lindner P, Scheper T, Reardon KF, Trends in Biotechnology, 31(7), 406 (2013)

[18] Shcolnick S, Shaked Y, Keren N, Biochim. Biophys. Acta (BBA)-Bioenergetics, 1767, 814 (2007)

[19] Zhang X, Liu S, Chen X, Ener. Procedia, 11, 2121 (2011)

[20] Liberton M, Berg RH, Heuser J, Roth R, Pakrasi HB, Protoplasma, 227, 129 (2006)