Production of Uniform Emulsion Droplets Using a Simple Fluidic Device with a Peristaltic Pump

Seok-Joon Hwang; Seung-Kwan Moon; Sung Eun Kim; Jung Hyun Kim; Sung-Wook Choi

Macromolecular Research, Vol.22, No.5, 557-561, May, 2014

DOI10.1007/s13233-014-2074-1 Export Citation

Production of Uniform Emulsion Droplets Using a Simple Fluidic Device with a Peristaltic Pump

Seok-Joon Hwang, Seung-Kwan Moon, Sung Eun Kim¹, Jung Hyun Kim², and Sung-Wook Choi^†

Department of Biotechnology, The Catholic University of Korea, Gyeonggi, 420-743, Korea
¹Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Seoul, 152-703, Korea
²Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, 120-749, Korea

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We fabricated a simple fluidic device consisting of a needle, a glass capillary, and a Tygon® tube to produce oil-in-water (O/W) emulsion droplets. In the fluidic device, the flow rate of the continuous phase was adjusted by a syringe or peristaltic pump, where a syringe pump was used for the discontinuous phase. We compared the performance of the syringe and peristaltic pump for the continuous phase in terms of the average diameter and coefficient of variance of the resultant O/W emulsion droplets. It was found that uniform droplets could be obtained when the production frequency was well matched. In addition, the flow behavior of the discontinuous phase during droplet formation was monitored using a high-speed camera, observing the back pressure in the peristaltic pump. By employment of a peristaltic pump for the continuous phase, it was possible to prepare a large amount of emulsion droplets.

Keywords:simple fluidic device;peristaltic pump;emulsion droplets;high-speed camera.

[References]

Langer R, Peppas NA, J. Macromol. Sci., Rev. Macromol. Chem. Phys., 23, 61 (1983)
Langer R, Science, 249, 1527 (1990)
Uhrich KE, Cannizzaro SM, Langer RS, Shakesheff KM, Chem. Rev., 99(11), 3181 (1999)
S. Margel and M. Offarim, Anal. Biochem., 128, 342 (1983)
Pittner F, Miron T, Pittner G, Wilchek M, J. Solid Phase Biochem., 5, 167 (1980)
Tokes ZA, Rogers KE, Rembaum A, Proc. Natl. Acad. Sci. U.S.A., 79, 2026 (1982)
Xu Q, Hashimoto M, Dang TT, Hoare T, Kohane DS, Whitesides GM, Langer R, Anderson DG, Small, 5, 1575 (2009)
Yang CH, Lin YS, Huang KS, Huang YC, Wang EC, Jhong JY, Kuo CY, Lab Chip, 9, 145 (2009)
Nisisako T, Okushima S, Torii T, Soft Matter, 1, 23 (2005)
Abate AR, Weitz DA, Small, 5, 2030 (2009)
Seo M, Paquet C, Nie ZH, Xu SQ, Kumacheva E, Soft Matter, 3, 986 (2007)
Choi SW, Cheong IW, Kim JH, Xia Y, Small, 5, 454 (2009)
Choi SW, Zhang Y, Xia YN, Adv. Funct. Mater., 19(18), 2943 (2009)
Choi SW, Yeh YC, Zhang Y, Sung HW, Xia Y, Small, 6, 1492 (2010)
Moon SK, Oh MJ, Paik DH, Ryu TK, Park K, Kim SE, Park JH, Kim JH, Choi SW, Macromol. Rapid Commun., 34(5), 399 (2013)
Eggers J, Rev. Mod. Phys., 69, 865 (1997)
Papageorgiou DT, Phys. Fluids, 7, 1529 (1995)
Rayleigh L, Proc. R. Soc. London, 29, 71 (1879)
Okushima S, Nisisako T, Torii T, Higuchi T, Langmuir, 20(23), 9905 (2004)

[Related Articles]

[1] Langer R, Peppas NA, J. Macromol. Sci., Rev. Macromol. Chem. Phys., 23, 61 (1983)

[2] Langer R, Science, 249, 1527 (1990)

[3] Uhrich KE, Cannizzaro SM, Langer RS, Shakesheff KM, Chem. Rev., 99(11), 3181 (1999)

[4] S. Margel and M. Offarim, Anal. Biochem., 128, 342 (1983)

[5] Pittner F, Miron T, Pittner G, Wilchek M, J. Solid Phase Biochem., 5, 167 (1980)

[6] Tokes ZA, Rogers KE, Rembaum A, Proc. Natl. Acad. Sci. U.S.A., 79, 2026 (1982)

[7] Xu Q, Hashimoto M, Dang TT, Hoare T, Kohane DS, Whitesides GM, Langer R, Anderson DG, Small, 5, 1575 (2009)

[8] Yang CH, Lin YS, Huang KS, Huang YC, Wang EC, Jhong JY, Kuo CY, Lab Chip, 9, 145 (2009)

[9] Nisisako T, Okushima S, Torii T, Soft Matter, 1, 23 (2005)

[10] Abate AR, Weitz DA, Small, 5, 2030 (2009)

[11] Seo M, Paquet C, Nie ZH, Xu SQ, Kumacheva E, Soft Matter, 3, 986 (2007)

[12] Choi SW, Cheong IW, Kim JH, Xia Y, Small, 5, 454 (2009)

[13] Choi SW, Zhang Y, Xia YN, Adv. Funct. Mater., 19(18), 2943 (2009)

[14] Choi SW, Yeh YC, Zhang Y, Sung HW, Xia Y, Small, 6, 1492 (2010)

[15] Moon SK, Oh MJ, Paik DH, Ryu TK, Park K, Kim SE, Park JH, Kim JH, Choi SW, Macromol. Rapid Commun., 34(5), 399 (2013)

[16] Eggers J, Rev. Mod. Phys., 69, 865 (1997)

[17] Papageorgiou DT, Phys. Fluids, 7, 1529 (1995)

[18] Rayleigh L, Proc. R. Soc. London, 29, 71 (1879)

[19] Okushima S, Nisisako T, Torii T, Higuchi T, Langmuir, 20(23), 9905 (2004)