Hemorheological changes and hematometric erythrocyte characteristics in rats after sodium nitrite intoxication

Ivan Ivanov; Yordanka Gluhcheva; Emilia Petrova; Nadia Antonova

Korea-Australia Rheology Journal, Vol.26, No.2, 225-228, May, 2014

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Hemorheological changes and hematometric erythrocyte characteristics in rats after sodium nitrite intoxication

Ivan Ivanov^†, Yordanka Gluhcheva¹, Emilia Petrova¹, and Nadia Antonova

Institute of Mechanics – BAS, Acad. G. Bonchev Str., Bl. 4, 1113 Sofia, Bulgaria
¹Institute of Experimental Morphology, Pathology and Anthropology with Museum – BAS, Acad. G. Bonchev Str., Bl. 25, 1113 Sofia, Bulgaria

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Sodium nitrite (NaNO2) is a precursor to a variety of organic compounds (pharmaceuticals, dyes and pesticides), but it is best known as a food additive. The aim of the study is to investigate the influence of acute (i.p.) treatment of Wistar rats with NaNO2 (at the dose of 50 mg/kg b.w.) on the blood rheological properties and erythrocyte hematometric indices (Hb, HCT, RBC, MCV, RDW, MCH, MCHC). The significant differences were not found in the whole blood viscosity (WBV) values of the control and NaNO2-treated groups. The changes in the erythrocyte hematometric indices were statistically significant for RDW, MCHC and MCH at the 1st hour, five- and ten days after NaNO2 administration. Interestingly, at the day 5th of the NaNO2 treatment we obtained statistically significant lower values for the RBC count, Hb, HCT, RDW, as well as elevated indices MCV (no statistically significant), MCH, MCHC. The results obtained indicate that hemorheological and hematometric parameters examined should be monitored in cases of acute exposure to nitrites - for the purposes of clinical toxicology. The quantitative values of hematometric indices reported in our experimental model could be suitable for predicting NaNO2 intoxication and methemoglobinemia in animals and humans.

Keywords:sodium nitrite;Wistar male rats;whole blood viscosity;hemorheology;erythrocyte hematometric indices

[References]

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Hirneth H, Classen HG, Arzneim-Forsch, 34, 988 (1984)
Imaizumi K, Tyuma I, Imai K, Kosada H, Udea Y, Int. Arch. Occup. Environ. Health, 45, 97 (1980)
Ivanov I, Antonova N, Gluhcheva Y, Petrova E, Ivanova Ju, J. Series on Biomechanics, 27, 45 (2012)
Jones DA, Andiapen M, Van-Eijl TJ, Webb AJ, Antoniou S, Schilling RJ, Ahluwalia A, Mathur A, BMJ Open 3, doi: 10.1136/bmjopen-2013-002813 (2013)
Kohn MC, Melnick RL, Ye F, Portier CJ, Drug Metab. Dispos., 30, 676 (2002)
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Rampling MW, Red cell aggregation and yield stress, Clinical Blood Rheology, vol. 1, G.D.O. Lowe, ed., CRC Press, Boca Radion, Florida, 45-64 (1988)
Reutov VP, Azhipa II, Kaiushin LP, Izv. Akad. Nauk SSSR Biol., 3, 408 (1983)
Savov Y, Antonova N, Zvetkova E, Gluhcheva Y, Ivanov I, Sainova I, Clin. Hemorheol. Microcirc., 35, 129 (2006)
Smith RP, Alkaitis AA, Shafer PR, Biochem. Pharmacol., 16, 317 (1967)

[1] Bartha JL, Comino-Delgado R, Bedoya FJ, Barahona M, Lubian D, Garcia-Benasach F, Eur. J. Obstet. Gynecol. Reprod. Biol., 82, 201 (1999)

[2] Doyle MP, Pickering RA, DeWeert TM, Hoekstra JW, Pater D, J. Biol. Chem., 256, 12393 (1981)

[3] FAO/WHO Food Additives Series 35, Geneva, World Health Organization, International Programme on Chemical Safety (1996)

[4] Han TH, Hyduke DR, Vaughn MW, Fukuto JM, Liao JC, Proc. Natl. Acad. Sci. USA, 99, 7763 (2002)

[5] Hirneth H, Classen HG, Arzneim-Forsch, 34, 988 (1984)

[6] Imaizumi K, Tyuma I, Imai K, Kosada H, Udea Y, Int. Arch. Occup. Environ. Health, 45, 97 (1980)

[7] Ivanov I, Antonova N, Gluhcheva Y, Petrova E, Ivanova Ju, J. Series on Biomechanics, 27, 45 (2012)

[8] Jones DA, Andiapen M, Van-Eijl TJ, Webb AJ, Antoniou S, Schilling RJ, Ahluwalia A, Mathur A, BMJ Open 3, doi: 10.1136/bmjopen-2013-002813 (2013)

[9] Kohn MC, Melnick RL, Ye F, Portier CJ, Drug Metab. Dispos., 30, 676 (2002)

[10] Magri CJ, Latib A, Chieffo A, Montorfano M, Colombo A, Eur. Heart J. 34, doi: 10.1093/eurheartj/eht310.P5419 (2013)

[11] National Toxicology Program, Natl. Toxicol. Program Tech. Rep. Ser., 495, 7 (2001)

[12] Novitskii VV, Ryazantseva NV, Shperling IA, Filippova ON, Rogov OA, Bull. Exp. Biol. Med., 142, 565 (2006)

[13] Rampling MW, Red cell aggregation and yield stress, Clinical Blood Rheology, vol. 1, G.D.O. Lowe, ed., CRC Press, Boca Radion, Florida, 45-64 (1988)

[14] Reutov VP, Azhipa II, Kaiushin LP, Izv. Akad. Nauk SSSR Biol., 3, 408 (1983)

[15] Savov Y, Antonova N, Zvetkova E, Gluhcheva Y, Ivanov I, Sainova I, Clin. Hemorheol. Microcirc., 35, 129 (2006)

[16] Smith RP, Alkaitis AA, Shafer PR, Biochem. Pharmacol., 16, 317 (1967)