Inhibition of Radical-Induced Oxidative DNA Damage by Antioxidants Loaded in Electrospun Polylactide Nanofibers

Elena Llorens; Luis J. del Valle; Jordi Puiggalí

Macromolecular Research, Vol.22, No.4, 388-396, April, 2014

DOI10.1007/s13233-014-2053-6 Export Citation

Inhibition of Radical-Induced Oxidative DNA Damage by Antioxidants Loaded in Electrospun Polylactide Nanofibers

Elena Llorens¹, Luis J. del Valle^{1, †}, and Jordi Puiggalí^{1, 2}

¹Departament d’Enginyeria Química, ETSEIB, Universitat Politècnica de Catalunya (UPC) Barcelona Tech, Avda. Diagonal 647, Barcelona, E-08028, Spain
²Center for Research in Nano-Engineering, Universitat Politècnica de Catalunya (UPC) Barcelona Tech, Campus Sud, Edifici C, C/Pasqual i Vila s/n, Barcelona, E-08028, Spain

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Polylactide (PLA) nanofibers loaded with antioxidants, i.e. vitamin B6 or pyridoxine (PN) and its analogue pyridoxal (PL), and with hydroxycinnamic acids, i.e. p-coumaric acid (CUM) and caffeic acid (CAF), were prepared by the electrospinning technique. The control consisted of nanofibers loaded with trolox (TRX). Experimentally, these new materials were tested on the inhibition of oxidative DNA damage caused by free radicals initiated by 2,2'-azobis (2-amidinopropane hydrochloride) (AAPH). This damage was assessed in vitro and in vivo by measuring the conversion of supercoiled pUC19 plasmid DNA to open circular and linear forms. It was found that these antioxidants, in solution, could significantly inhibit oxidative DNA damage, and that the antioxidants loaded in the PLA nanofibers maintained in vitro and in vivo their protective role against oxidative DNA damage. Thus, electrospun mats derived from PLA nanofibers loaded with the considered compounds have the capacity to protect DNA against oxidative damage, and appear interesting, for their use in the purification of plasmidic or genomic DNA.

Keywords:electrospinning;polylactide nanofiber;antioxidant;oxidative damage;DNA damage.

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[Referenced By]

[1] Finkel T, Holbrook NJ, Nature, 408, 239 (2000)

[2] Barnham KJ, Masters CL, Bush AI, Nat. Rev. Drug Discov., 3, 205 (2004)

[3] Hussain SP, Hofseth LJ, Harris CC, Nat. Rev. Cancer, 3, 276 (2003)

[4] Cooke MS, Evans MD, Dizdaroglu M, Lunec J, Faseb J., 17, 1195 (2003)

[5] Pizarro JG, Folch J, Vazquez de la Torre A, Verdaguer E, Junyent F, Jordan J, Pallas M, Camins A, Free Radic. Res., 43, 985 (2009)

[6] Pero RW, Anderson MW, Doyle GA, Anna CH, Romagna F, Markowitz M, Bryngelsson C, Cancer Res., 50, 4619 (1990)

[7] Brash DE, Harve PA, Proc. Natl. Acad. Sci. U.S.A., 99, 13969 (2002)

[8] Rice-Evans CA, Diploc AT, Free Radic. Biol. Med., 15, 77 (1993)

[9] Surh YJ, Nat. Rev. Cancer, 3, 768 (2003)

[10] Shibata K, Mushiage M, Kondo T, Hayakawa T, Tsuge H, Biosci., Biotechnol. Biochem., 59, 2060 (1995)

[11] Bruce NA, Mutat. Res., 475, 7 (2001)

[12] Larsson SC, Giovannucci E, Wolk A, Gastroenterology, 128, 1830 (2005)

[13] Natori Y, Oka T, Nutr. Res., 17, 1199 (1997)

[14] Matxain JM, Ristila M, Strid A, Eriksson LA, J. Phys. Chem. A, 110(48), 13068 (2006)

[15] Pino E, Campos AM, Lopez-Alarcon C, Aspee A, Lissi E, J. Phys. Org. Chem., 19, 759 (2006)

[16] Sill TJ, von Recum HA, Biomaterials, 29, 1989 (2008)

[17] Cui W, Chang J, Dalton PD, Biomaterials, 33, 445 (2011)

[18] Gorsline RT, Tangkawattana P, Lannutti JJ, Yamaguchi M, Kaeding CC, Bertone AL, J. Biomed. Sci. Eng., 3, 908 (2010)

[19] Li WJ, Laurencin CT, Caterson EJ, Tuan RS, Ko FK, J. Biomed. Mater. Res., 60, 613 (2002)

[20] Luu YK, Kim K, Hsiao BS, Chu B, Hadjiargyrou M, J. Control. Release, 89, 341 (2003)

[21] Llorens E, del Valle LJ, Diaz A, Casas MT, Puiggali J, Macromol. Res., 21(7), 775 (2013)

[22] Wei QY, Zhou B, Cai YJ, Yang L, Liu ZL, Food Chem., 96, 90 (2006)

[23] Burger C, Hsiao BS, Chu B, Annu. Rev. Mater. Res., 36, 333 (2006)

[24] del Valle LJ, Camps R, Diaz A, Franco L, Rodriguez-Galan A, Puiggali J, J. Polym. Res., 18, 1903 (2011)

[25] del Valle LJ, Roa M, Diaz A, Casas MT, Puiggali J, Rodriguez-Galan A, J. Polym. Res., 19, 9792 (2012)

[26] Ribeiro C, Sencadas V, Costa CM, Gomez Ribelles JL, Lanceros-Mendez S, Sci. Technol. Adv. Mater., 12, 015001 (2011)

[27] Kevadiya BD, Joshi GV, Patel HA, Ingole PG, Mody HM, Bajaj HC, J. Biomater. Appl., 25, 161 (2009)

[28] Bartl F, Urjasz H, Brzezinski B, J. Mol. Struct., 441, 77 (1998)

[29] Casas JS, Castineiras A, Condori F, Couce MD, Russo U, Sanchez A, Sordo J, Varela JM, Polyhedron, 19, 813 (2000)

[30] Swislocka R, Kowczyk-Sadowy M, Kalinowska M, Lewandowski W, Spectroscopy, 27, 35 (2012)

[31] Xing Y, Peng HY, Zhang MX, Li X, Zeng WW, Yang XE, J. Zhejiang Univ. Sci. B, 13, 487 (2012)

[32] Gastaldi L, Ugazio E, Sapino S, Iliade P, Miletto I, Berlier G, Phys. Chem. Chem. Phys., 14, 11318 (2012)

[33] Teixeira-Dias B, del Valle LJ, Estrany F, Armelin E, Olive R, Aleman C, Eur. Polym. J., 44, 3700 (2008)

[34] Aleman C, Teixeira-Dias B, Zanuy D, Estrany F, Armelin E, del Valle LJ, Polymer, 50(8), 1965 (2009)

[35] Zhang P, Omaye ST, Food Chem. Toxicol., 39, 239 (2001)

[36] Liu SQ, Cao ML, Dong SL, Bioelectrochemistry, 74, 164 (2008)

[37] Liu SQ, Sun WH, Li LC, Li H, Wang XL, Int. J. Electrochem. Sci., 7, 324 (2012)