In situ Sodium Alginate-Hyaluronic Acid Hydrogel Coating Method for Clinical Applications

Ying Liu; Li Jie Duan; Min Ji Kim; Ji-Heung Kim; Dong June Chung

Macromolecular Research, Vol.22, No.3, 240-247, March, 2014

DOI10.1007/s13233-014-2001-5 Export Citation

In situ Sodium Alginate-Hyaluronic Acid Hydrogel Coating Method for Clinical Applications

Ying Liu¹, Li Jie Duan^{1, 2}, Min Ji Kim¹, Ji-Heung Kim³, and Dong June Chung^{1, †}

¹Department of Polymer Science and Engineering, Sungkyunkwan University, Gyeonggi, 440-746, Korea
²School of Chemistry and Life Science, Changchun University of Technology, Changchun, 130022, P. R. China
³Department of Chemical Engineering, Sungkyunkwan University, Gyeonggi, 440-746, Korea

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A novel synthetic method is reported to prepare HAAL hydrogels derived from hyaluronic acid (HA) and sodium alginate (AL) with different compositions (ranging from 1:1 to 1:5) through ionic and covalent crosslinking is reported in this study. The synthesized hydrogels were characterized by FTIR spectroscopy. Surface morphology and equilibrium swelling behavior of the hydrogels were also examined. In an MTT assay, the hydrogel extracts were found to be non-cytotoxic to L929 mouse fibroblasts. The blood compatibility of the synthesized hydrogels was assayed with a platelet adhesion test, and was comparable with that of medical-grade polyurethane (PU, Pellethane®). HAAL hydrogels with recombinant human epidermal growth factor (rh-EGF) exhibited enhanced cell proliferation and adhesion (human L929 fibroblast). These characteristics demonstrate that HAAL hydrogels treated using this in situ coating method have potential for usage in various clinical applications.

Keywords:sodium alginate;hyaluronic acid;hydrogel;in situ coating;non-toxic;recombinant human epidermal growth factor (rh-EGF).

[References]

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Marques AP, Reis RL, Hunt JA, Biomaterials., 23, 1471 (2002)
Serrano MC, Pagani R, Vallet-Regi M, Pena J, Ramila A, Izquierdo I, Portoles MT, Biomaterials., 25, 5603 (2004)
Kim YS, Lew DH, Tark KC, Rah DK, Hong JP, J. Korean Med. Sci., 25, 589 (2010)
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Bajpai SK, Sharma S, React. Funct. Polym., 59, 129 (2004)
Mazuder R, Nath L, Haque A, Maity T, Choudhury PK, Shrestha B, Chakranorty M, Pal RN, Int. J. Pharm.Pharm. Sci., 2, 211 (2010)
Chung CW, Kang JY, Yoon IS, Hwang HD, Balakrishnan P, Cho HJ, Chung KD, Kang DH, Kim DD, Colloids Surf. B: Biointerface., 88, 711 (2011)
Ratner BD, Hoffman AS, Hanson SR, Harker LA, Whiffen JD, J. Polym. Sci. Part C: Polym. Symp., 66, 363 (1979)

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[1] Yang TL, Yoo JJ, Siemionow MZ, Atala A, in The Know-How of Face Transplantation, Springer, London, Part 7, 447 (2011)

[2] Nam HS, An J, Chung DJ, Kim JH, Chung CP, Macromol. Res., 14(5), 530 (2006)

[3] Lee CM, Jeong HJ, Kim DW, Lee KY, Macromol. Res., 16(5), 429 (2008)

[4] Yoo HS, Kim TG, Park TG, Adv. Drug Deliv. Rev., 61, 1033 (2009)

[5] Francis Suh JK, Matthew HWT, Biomaterials., 21, 2589 (2000)

[6] Leach JB, Bivens KA, Patrick CW, Schmidt CE, Biotechnol. Bioeng., 82(5), 578 (2003)

[7] Rubinstein A, Nakar D, Sintov A, Int. J. Pharm., 84, 141 (1992)

[8] Ford MC, Bertram JP, Hynes SR, Michaud M, Li Q, Young M, Segal SS, Madri JA, Lavik EB, Proc.Natl. Acad. Sci., 103, 2512 (2006)

[9] Chen LG, Liu ZL, Zhuo RX, Polymer, 46(16), 6274 (2005)

[10] Mosmann T, J. Immunol. Methods., 65, 55 (1983)

[11] Sgouras D, Duncan R, J. Mater. Sci. Mater. Med., 1, 61 (1990)

[12] Hansen MB, Nielsen SE, Berg K, J. Immunol. Methods., 119, 203 (1989)

[13] Marques AP, Reis RL, Hunt JA, Biomaterials., 23, 1471 (2002)

[14] Serrano MC, Pagani R, Vallet-Regi M, Pena J, Ramila A, Izquierdo I, Portoles MT, Biomaterials., 25, 5603 (2004)

[15] Kim YS, Lew DH, Tark KC, Rah DK, Hong JP, J. Korean Med. Sci., 25, 589 (2010)

[16] Brown GL, Nanney LB, Griffen J, Cramer AB, Yancey JM, Curtsinger LJ, Holtzin L, Schultz GS, Jurkiewicz MJ, Lynch JB, N. Engl. J. Med., 321, 76 (1989)

[17] Taylor JM, Mitchell WM, Cohen S, J. Biol. Chem., 247, 5928 (1972)

[18] Zhang L, Xiao Y, Jiang B, Fan H, Zhang X, J. Biomed.Mater. Res. Part B: Appl. Biomater., 92B, 307 (2010)

[19] Lei J, Kim JH, Jeon YS, Macromol. Res., 16(1), 45 (2008)

[20] Bajpai SK, Sharma S, React. Funct. Polym., 59, 129 (2004)

[21] Mazuder R, Nath L, Haque A, Maity T, Choudhury PK, Shrestha B, Chakranorty M, Pal RN, Int. J. Pharm.Pharm. Sci., 2, 211 (2010)

[22] Chung CW, Kang JY, Yoon IS, Hwang HD, Balakrishnan P, Cho HJ, Chung KD, Kang DH, Kim DD, Colloids Surf. B: Biointerface., 88, 711 (2011)

[23] Ratner BD, Hoffman AS, Hanson SR, Harker LA, Whiffen JD, J. Polym. Sci. Part C: Polym. Symp., 66, 363 (1979)