수용액상에서의 키토산 구조에 대한 분광학적 연구

하병조; 유병희; 이옥섭; 이윤식

Journal of the Korean Industrial and Engineering Chemistry, Vol.6, No.6, 1038-1044, December, 1995

Export Citation

수용액상에서의 키토산 구조에 대한 분광학적 연구

Spectrophotometric Investigation of Chitosan Structure in Aqueous Solution

하병조, 유병희, 이옥섭, 이윤식

초록

수용액상에서 키토산의 구조변화를 UV spectra 및 CD spectra를 이용하여 연구하였다. 요오드와 키토산의 상호작용을 UV spectra로 관찰한 결과 pH 감소에 따라 246nm에서의 새로운 상호작용 peak가 증가되었으며, 이는 NaCl의 농도가 증가함에 따라 나타나는 246nm의 상호작용 peak와 일치하였다. 그러나 urea의 농도를 변화시킨 경우에는 246nm의 상호작용 peak가 관찰되지 않았다. 또한 CD spectra에서는 키토산용액의 경우 197nm에서 강한 양의 Cotton효과를 나타내었으며, 208nm 및 222nm에서 강한 음의 Cotton 효과를 보였다. 이러한 peak들은 NaCl의 농도를 증가시켜 줌에따라 그 세기가 감소하는 경향을 나타내었다.

For the study of structural change of chitosan which is dissolved in aqueous solution, UV spectra and CD spectra were used. The interaction between chitosan and iodine observed in UV spectra shows that new interaction peak at 246nm increases as the pH decreases, which is also observed as NaCl concentration increases. But there is no observable peak at 246nm when the concentration of urea is varied. CD spectra of chitosan solution reveals the strong positive Cotton effect at 197nm, and strong negative Cotton effects at 208nm, 222nm. As the concentration of NaCl increases, the intensity of these peaks decreases.

[References]

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[1] Choi KS, Macromol. Chem. Macromol. Symp., 33, 55 (1990)

[2] Kim JH, Lee YM, Polymer, 34, 1952 (1993)

[3] Lee YM, Shin EM, Yang KS, Polym.(Korea), 15(2), 182 (1991)

[4] Muzzarelli RAA, "Chitin," Pergamon Press, New York (1977)

[5] Clark GL, Smith AF, J. Phys. Chem., 40, 863 (1936)

[6] Saito H, Tabeta R, Ogawa K, Macromolecules, 20, 2424 (1987)

[7] Ogawa K, Agric. Biol. Chem., 55, 2375 (1991)

[8] Ogawa K, Inukai S, Carbohydr. Res., 160, 425 (1987)

[9] Bluhm TL, Sarko A, Carbohydr. Res., 54, 125 (1977)

[10] Glass JE, "Water-Soluble Polymers," Maple Press Company, New York PA (1986)

[11] Grant S, Blair HS, Mckay G, Macromol. Chem., 190, 2279 (1989)

[12] Cairns P, Miles MJ, Morris VJ, Ridout MT, Brownsey GJ, Winter WT, Carbohydr. Res., 235, 23 (1992)

[13] Ogawa K, Inukai S, Carbohydr. Res., 160, 425 (1987)

[14] Ono S, Tsuchihashi S, Kuge T, J. Am. Chem. Soc., 75, 3601 (1953)

[15] Nishimura T, Yajima H, Kubota S, Ishii T, Endo R, Kobunshi Ronbunshu, 45, 945 (1988)

[16] Nishimura T, Yajima H, Kubota S, Ishii T, Endo R, Kobunshi Ronbunshu, 47, 717 (1990)

[17] Stone AL, Biopolymers, 10, 739 (1971)

[18] Rutherford FA, Austin PR, Proc. Int. Conf. Chitin/Chitosan 1st, 182 (1978)

[19] Sannan T, Kukita K, Ogawa K, Iwakura Y, Polymer, 19, 458 (1979)

[20] Pal MK, Pal PK, Makromol. Chem., 190, 2929 (1989)

[21] Yokota T, Kimura Y, Makromol. Chem., 194, 295 (1993)

[22] Pal MK, Pal PK, Biopolymers, 29, 751 (1990)

[23] Yui T, Kobayashi H, Kitamura S, Imada K, Biopolymers, 34, 203 (1994)

[24] Scaria PV, Atreyi M, Rao MV, Biopolymers, 25, 2349 (1986)

[25] Matsumoto T, Kawai M, Masuda T, Biopolymers, 31, 1721 (1991)

[26] Coduti PL, Gordon EC, Bush CA, Anal. Biochem., 78, 9 (1977)

[27] Hasegawa M, Isogai A, Onabe F, Usuda M, J. Appl. Polym. Sci., 45, 1857 (1992)

[28] Park JW, Chakrabarti B, Biochim. Biophys. Acta, 544, 667 (1978)

[29] Morris ER, Rees DA, Snaderson GR, Thom D, J. Chem. Soc.-Perkin Trans. 2, 1418 (1975)