Adhesion to substrate and antimicrobial efficiency of pulsed laser-deposited ZnO nanostructures were significantly increased by interposing a buffer nano-layer of hydrophobin. The hydrophobin interlayer increased by eight times the ZnO film resilience to wash in water, while in alkaline or acidic artificial sweat, it increased by 2 and 1.2 times, respectively, as compared to textiles covered with ZnO films only. Hydrophobin boosted the biocide effect of ZnO nanostructured films in case of Candida albicans and mold mix inoculum cultures by 50 and 30 %, respectively. An interpretation of these phenomena is advanced based upon the results of the structural investigations.