농도 차이가 큰 두 물질의 크로마토그래피를 이용한 분리

노경호

Journal of the Korean Industrial and Engineering Chemistry, Vol.6, No.2, 193-199, April, 1995

Export Citation

농도 차이가 큰 두 물질의 크로마토그래피를 이용한 분리

Separation of Two Components in the Case of Large Difference in Concentration by Chromatography

노경호

초록

Thymine monomer가 UV에 노출되면 미량의 thymine dimer가 존재하며 이는 발암물질로 알려져 있다. Thymine monomer와 dimer는 농도 차이가 크기 때문에 두 물질의 농도 비에 따른 정확한 정량 및 정성분석을 하는 것이 중요하다. 이를 위해서 역상 고성능 액체 크로마토그래피(RP-HPLC)를 이용하여 두 물질의 Langmuir isoterm을 실험적으로 구하였고 비선형 수학적 모델을 이용하여 thymine dimer와 monomer의 농도 분포 곡선을 예측하였다 두 물질의 농도비와 시료의 양에 따른 예측된 농도 분포 곡선에서 thymine dimer의 정량 및 정성분석이 가능하게 되었으며 각각의 물질을 yield로 표시하여 순수하게 얻을 수 있는 영역을 제시하였다.

By a UV exposure of thymine monomer, a very small amount of thymine dimer exists and it is reported the dimer causes the cancer. Because of large differences in their concentrations, it is important that the accurate quantitative and qualititive analysis is highly required with their concentration ratios. The equilibrium isotherm of the two components, expressed as the Langmuir type, was experimentally measured by reveresed-phase high performance liquid chromatography( RP-HPLC) and, based on the nonlinear mathematical model, their concentration profiles were calculated for the prediction of their behaviors in the chromatographic column. By the predicted concentration profiles with the change in the sample size and concentration ratio of the two components, the band of the dimer can be known. And a time domain where each component could be collected as a cutting fraction was examined from the yield calculations.

[References]

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[1] Barker PE, "Developments in Chromatography," Knapman C.H. (Editor), Applied Science Publisher, London (1978)

[2] Grushka E, "Preparative-Scale Chromatography," Marcel Dekker, Inc., New York and Basel (1989)

[3] Row KH, Lee WK, "Separation by Gas-Liquid Chromatography," Cheremisinoff, N.P. (Editor), Handbook of Heat and Mass Transfer, 3: Catalysis, Kinetics, and Reactor Engineering, Chapter 22, Gulf Publishing Company, Houston (1989)

[4] Hamilton RJ, Sewell PA, "Introduction to High Performance Liquid Chromatography," 2nd Ed., Chapman and Hall, London (1982)

[5] Row KH, Chem. Ind. Technol., 7(2), 162 (1989)

[6] Cadet J, Gentner NE, Paterson MC, J. Chromatogr., 280, 99 (1983)

[7] Love JD, Friedberg EC, J. Chromatogr., 240, 475 (1982)

[8] Rouchon P, Valentine P, Schonauer M, Guichon G, Sep. Sci. Technol., 22, 1793 (1987)

[9] Jaulmes A, Vidal-Madjar C, Colin H, Guichon G, J. Phys. Chem., 90, 207 (1986)

[10] Guiochon G, Ghodbane S, J. Phys. Chem., 92, 3682 (1988)

[11] Ghodbane S, Guiochon G, J. Chromatogr., 450, 27 (1988)

[12] Wang SY, Nature, 190, 690 (1961)

[13] Row KH, Choi DK, Lee YY, Korean J. Chem. Eng., 7(2), 151 (1990)

[14] Katti AM, Ramsey R, Guiochon G, J. Chromatogr., 477, 119 (1989)