The extraction behavior of Eu(III) and Am(III) in a solution of bis(2-ethylhexyl)diglycolamic acid (HDEHDGA) in n-dodecane (n-DD) from citric acid (CA) medium was studied as a function of various parameters. The extraction increased with increase of pH, reached a maximum at pH 2 followed by decrease. The stripping behavior of Eu(III) and Am(III) from the loaded organic phase was studied by using a solution of diethylenetriaminepentaacetic acid (DTPA) and CA. The conditions needed for the efficient separation of Am(III) from Eu(III) were optimized. Based on the optimized conditions, the feasibility of separating Am(III) from Eu(III) present in CA feed solution was investigated in a 20- stage mixer-settler. Quantitative extraction of Eu(III) and Am(III) in 0.1M HDEHDGA/n-DD was achieved in 3-4 stages, whereas the selective back extraction of Am(III) was achieved in approximate to 20 stages upon contacting the loaded organic phase with a stripping formulation composed of DTPA-CA at pH 1.5. The results confirmed the possibility of using diglycolamic acid for the separation of trivalent actinides from the chemically similar lanthanides, which is indeed necessary for transmutation of minor actinides present in high-level liquid waste (HLLW).