Journal of the Korean Industrial and Engineering Chemistry, Vol.5, No.2, 321-326, April, 1994
포스파젠 고분자의 친수성 겔을 이용한 효소의 고정
Enzyme Immobilization with Polyphosphazene Hydrogels
초록
에테르기를 가진 수용성 포스파젠 고분자를 감마광선에 노출시켜 좋은 수분-팽윤도를 가진 친수성 겔을 만들었다. 이 친수성 겔들의 물리적 강도가 감마광선의 강도에 따라 조절될 수 있었다. 트립신과 수용성 포스파젠 고분자를 함께 감마광선에 쪼여 트립신을 겔의 망 사이로 고정시켰다.
고정된 트립신의 활성도는 N-α-benzoyl-1-arginine-p-nitroanilide와 반응시킨 후 분광학적인 방법으로 조사하였다. 고정된 트립신은 적어도 500시간 후에도 상당히 좋은 활성도 수율과 안정도를 가지고 있었음을 보여주었다.
고정된 트립신의 활성도는 N-α-benzoyl-1-arginine-p-nitroanilide와 반응시킨 후 분광학적인 방법으로 조사하였다. 고정된 트립신은 적어도 500시간 후에도 상당히 좋은 활성도 수율과 안정도를 가지고 있었음을 보여주었다.
The water-soluble polyphosphazene possessing ether side groups was exposed to γ-rays to prepare hydrogens with good water-swellability. The physical strength of these hydrogels could be controlled by irradiation dose of γ-rays. Trypsin and water-soluble polyphosphazene were irradiated together by γ-rays for entrapment of enzymes into hydrogel networks.
The activities of immobilized trypsin were examined spectrophotometrically after the reaction with N-α-benzoyl-1-arginine-p-nitroanilide in phosphate buffer. The immobilized trypsin was found to have good activity yield and suability after at least 500 hours.
The activities of immobilized trypsin were examined spectrophotometrically after the reaction with N-α-benzoyl-1-arginine-p-nitroanilide in phosphate buffer. The immobilized trypsin was found to have good activity yield and suability after at least 500 hours.
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