The technique of enzyme treatment on the water-soluble proteins and mechanical properties of natural rubber latex (NRL) films was studied. The main aim was to introduce an enzymic catalysis method to tackle the protein allergy problem in NRL product. The suitable pH value, the temperature, and the best proportion of transglutaminase to deal with NRL were found. The protein spillage of modified NRL films was greatly lower than that of unmodified NRL, and the tensile strength and the elongation at break of the modified NRL films almost had been scarcely changed. The compactness of the NRL films was improved simultaneously. These suggest that the modified NRL can be used as a kind of latex with low-allergy personal barrier products such as surgical gloves. (c) 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013