We report on a green procedure for the synthesis and stabilization of gold nanoparticles (AuNPs) from chlorauric acid (HAuCl4) with the use of a beta-glucan known as Lentinan (LNT) without external reducing or stabilizing agents in aqueous medium. LNT adopted triple helical conformation in water, which was first denatured into single chains (s-LNT) at a high temperature of 140 degrees C before mixing with HAuCl4. Results from UV-vis absorption spectroscopy, transmission electron microscopy (TEM), and energy dispersive X-ray (EDX) spectra suggested that AuCl4- was rapidly reduced to AuNPs by s-LNT. Moreover, the as-prepared AuNPs could be converted into nanobelt, spherical nanoparticles, and nanowire morphology simply by controlling the s-LNT concentration, reaction time, and temperature. In particular, the AuNPs nanowire was confirmed as the most stable shape in water, which was predominately ascribed to the hydrophobic cavity in the helical center of the renatured triple helical LNT (r-LNT) from s-LNT. Namely, AuNPs were entrapped in the hydrophobic cavity of r-LNT to form nanowire with an outer layer of water-soluble r-LNT, leading to stable dispersion of AuNPs. All the data demonstrated that the beta-glucan of s-LNT can be used as a reducing and stabilizing agent to synthesize and disperse AuNPs in water. The whole process of reduction and stabilization was free of organic solvent and thus very safe, which is important for the potential application of AuNPs in biotechnology and biomedicine.