Evaluations of chitosan/poly(D,L-lactic-co-glycolic acid) composite fibrous scaffold for tissue applications

Soo Jung Kim; Dae Hyeok Yang; Heung Jae Chun; Gue Tae Chae; Ju Woong Jang; Young Bock Shim

Macromolecular Research, Vol.21, No.8, 931-939, August, 2013

DOI10.1007/s13233-013-1110-x Export Citation

Evaluations of chitosan/poly(D,L-lactic-co-glycolic acid) composite fibrous scaffold for tissue applications

Soo Jung Kim^{1, 2}, Dae Hyeok Yang^{1, 3}, Heung Jae Chun^{1, 2, †}, Gue Tae Chae⁴, Ju Woong Jang⁴, and Young Bock Shim⁴

¹Institute of Cell & Tissue Engineering, The Catholic University of Korea, Catholic University of Korea, Seoul, 137-701, Korea
²Department of Biomedical Sciences, The Catholic University of Korea, Seoul, 137-701, Korea
³, Korea
⁴Institute of Hansen’s Disease, College of Medicine, The Catholic University of Korea, Seoul, 137-701, Korea

E-mail:

The objective of the present study was to evaluate non-woven chitosan/poly(lactic-co-glycolic acid) fibrous scaffold (chitosan/PLGA FS) prepared by a co-electrospinning process for tissue engineering applications, as compared to chitosan and PLGA FSs. The morphological, structural, and mechanical properties of the FSs were assessed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy-attenuated total reflection mode (FTIR-ATR), and a universal testing machine (UTM). The biocompatibility of the FSs was also evaluated in vitro in cultures of mouse fibroblasts and in vivo by subcutaneous implantation studies in rats. SEM image of the chitosan/PLGA FS showed morphological similarities to the natural ECM, characterized by high surface-to-volume ratio, high porosity, and variable pore size distributions. The FTIR-ATR spectrum of chitosan/PLGA FS revealed incorporation of the characteristic bands of chitosan and PLGA, indicating the co-existence of two fibrous structures. The poor mechanical properties of chitosan FS was improved by co-electrospinning with PLGA. In vitro L929 cell proliferation assay revealed that the cytocompatibility of chitosan/PLGA FS was increased compared to that of PLGA. In addition, chitosan/PLGA FS showed the improved ability to resolve foreign body reactions compared to PLGA FS due to the presence of chitosan.

Keywords:chitosan;poly(lactic-co-glycolic acid);co-electrospinning;non-woven fibrous scaffold;cell proliferation;foreign body reaction

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[Referenced By]

[1] Woo KM, Chen VJ, Ma PX, J. Biomed. Mater. Res. A, 2, 531 (2003)

[2] da Silva MLA, Crawford A, Mundy JM, Correlo VM, Sol P, Bhattacharya M, Hatton PV, Reis RL, Neves NM, Acta Biomater., 6, 1149 (2010)

[3] Cai Q, Wan Y, Bei J, Wang S, Biomaterials, 24, 3555 (2003)

[4] Astete CE, Sabliov CM, J. Biomater. Sci.-Polym. Ed., 17, 247 (2006)

[5] Xie D, Huang H, Blackwood K, MacNeil S, Biomed. Mater., 5, 065016 (2010)

[6] Anderson J, Shive M, Adv. Drug Deliv. Rev., 28, 5 (1997)

[7] Pinho ED, Martins A, Araujo JV, Reis RL, Neves NM, Acta Biomater., 5, 1104 (2009)

[8] Chun HJ, Kim GW, Kim CH, J. Phys. Chem. Solids, 69, 1573 (2008)

[9] Muzzarelli RAA, Greco F, Busilacchi A, Sollazzo V, Gigante A, Carbohydr. Polym., 89, 723 (2012)

[10] Cooper A, Bhattarai N, Zhang M, Carbohydr. Polym., 85, 149 (2011)

[11] Seo NM, Ko JH, Park YH, Chun HJ, J. Tissue Eng. Regen. Med., 8, 16 (2011)

[12] Huang ZM, Zhang YZ, Kotaki M, Ramakrishna S, Compos. Sci. Technol., 63, 2223 (2003)

[13] Sangsanoh P, Supaphol P, Biomacromolecules, 7(10), 2710 (2006)

[14] Jayakumar R, Prabaharan M, Nair SV, Tamura H, Biotechnol. Adv., 28, 142 (2010)

[15] Sangsanoh P, Supaphol P, Biomacromolecules, 7(10), 2710 (2006)

[16] Hasegawa M, Isogai A, Onabe F, Usuda M, J. Appl. Polym. Sci., 45, 1857 (1992)

[17] Ngiam M, Liao S, Patil AJ, Cheng Z, Chan CK, Ramakrishna S, Bone, 45, 4 (2009)

[18] Cheng M, Deng J, Yang F, Gong Y, Zhao N, Zhang X, Biomaterials, 24, 2871 (2003)

[19] Meng ZX, Wang YS, Ma C, Zheng W, Li L, Zheng YF, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 30, 1204 (2010)

[20] Gupta D, Venugopal J, Mitra S, Dev VRG, Ramakrishna S, Biomaterials, 30, 2085 (2009)

[21] Semete B, Booysen LIJ, Kalombo L, Venter JD, Katata L, Ramalapa B, Verschoor JA, Swai H, Toxicol. Appl. Pharm., 249, 158 (2010)

[22] Jeon DW, Ahn WS, You SJ, Chae GT, Shim YB, Chun HJ, Appl. Surf. Sci., 262, 134 (2012)

[23] Fournier E, Passirani C, Montero-Menei CN, Benoit JP, Biomaterials, 24, 3311 (2003)

[24] Park H, Lee KY, Lee SJ, Park KE, Park WH, Macromol. Res., 15(3), 238 (2007)

[25] Park HN, Lee JB, Kwon IK, Int. J. Tissue Regen., 1, 10 (2010)

[26] Waring MJ, Parsons D, Biomaterials, 22, 903 (2001)

[27] Dai T, Tanaka M, Huang YY, Hamblin MR, Expert Rev. Anti Infect. Ther., 9, 857 (2011)

[28] Aderem A, Underhill DM, Annu. Rev. Immunol., 17, 593 (1999)

[29] Anderson JM, in Tissue Engineering of Vascular Prosthetic Grafts, Xilla P, Greisler HP, Landes RG, Eds., Landes Biosciense, Cape Town, 1999, pp 197-206.

[30] Anderson JM, Annu. Rev. Mater. Res., 31, 81 (2001)

[31] Gordon S, Nat. Rev. Immunol., 3, 23 (2003)

[32] Ma J, Chen J, Mandelin J, Ceponis A, Miller NE, Hukkanen M, Konttinen YT, Cell. Mol. Life Sci., 60, 2334 (2003)