은-알지네이트/PVP 나노섬유의 항균 특성

최유성; 민경두; 윤두수; Yoo-Sung Choi; Kyung-Du Min; Doo-Soo Yoon

Applied Chemistry for Engineering, Vol.24, No.4, 416-422, August, 2013

Export Citation

은-알지네이트/PVP 나노섬유의 항균 특성

Antibacterial Properties of Silver-alginate/PVP Nanofiber

최유성, 민경두, 윤두수^{1, †}

(주)럭키산업
¹조선이공대학교 생명환경화공과

Yoo-Sung Choi, Kyung-Du Min, and Doo-Soo Yoon^{1, †}

R&D team, Lucky Industry, Gwangju 506-250, Korea
¹Department of Bioenvironmental & Chemical Engineering, Chosun College of Science & Technology, Gwang-ju 501-744, Korea

E-mail:

초록

알지네이트에 은이온을 첨가하기 위하여, 질산은 수용액을 이용하여 은-알지네이트를 제조하였다. 본 연구에서는 은-알지네이트를 Poly vinylpyrrolidone (PVP) 수용액과 블렌드하였고, 전기방사는 블렌드 용액을 이용하여 수행하였다. 은-알지네이트/PVP 혼합 용액의 항균효과는 colony counting test로 대장균과 포도상 구균에 대해 확인하였다. 은-알 지네이트/PVP 혼합용액의 전기방사 조건은 조성물의 농도를 다양하게 하여 방사거리 22 cm, 방사속도 0.01 mL/min, 전압 26 kV 조건하에서 수행하여 나노섬유를 제조하였다. 은-알지네이트 나노 섬유의 형태와 크기는 SEM과 Image J를 통해 확인하였으며, 전기방사된 SA5P15 섬유들의 평균 직경은 124 nm를 보였으며, 균일하게 방사되는 것을 확인하였다. SA5P15의 균 감소율은 24 시간 후 99.9%를 보였다.

In order to incorporate silver ions into the alginate, silver-alginate was prepared with aqueous solutions of silver nitrate. In the study, the silver-alginate was prepared by blending with poly vinylpyrrolidone solutions and the electrospinning was performed by using this blend solution. Antibacterial properties of silver-alginate/PVP solutions were estimated for Escherichia coli and Staphylococcus aureus by the colony counting test. Electrospinning conditions of silver-alginate/PVP solution were the tip-to-collector distance of 22 cm, the flow rate of the solution at 0.01 mL/min, and the voltage at 26 kV. The form and size of silver-alginate/PVP nanofibers were estimated by SEM and Image J. The average diameter of the electrospun SA5P15 fibers was 124 nm and showed a narrow diameter distribution. The reduction of bacteria for SA5P15 exhibited 99.9% after 24 h.

Keywords:silver-alginate;low molecular weight sodium alginate;electrospun;poly vinylpyrrolidone

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[2] Nishide E, Hiroshi A, Naoyuki U, Nippon Suisan Gakkaishi., 53, 1215 (1987)

[3] Fujihara M, Nagumo T, J. Chromatogr., A., 465, 386 (1989)

[4] Noda K, Takada K, Bull. J. Soc. Sci. Fish., 49, 1591 (1983)

[5] Han SM, Nam CW, Ko SW, J. Kor. Fiber Soc., 37, 365 (2000)

[6] Qin YM, J. Appl. Polym. Sci., 91(3), 1641 (2004)

[7] Eltahlawy KF, Elbelndary MA, Elhendawy AG, Hudson SM, Carbohydr. Polym., 60, 421 (2005)

[8] Ma M, Sun G, Dyes Pigm., 66, 33 (2005)

[9] Lee JW, Broughton RM, Akdag A, Worley SD, Huang TS, Fibers Polym., 8, 148 (2007)

[10] Qin Y, Polym. Adv., Technol., 19, 6 (2008)

[11] Liu Y, Chen J, Misoska V, Wallance GG, Reac. Funct.Polym., 67, 461 (2007)

[12] Park K, Park S, Kim G, Kim W, Polym.(Korea), 32(3), 206 (2008)

[13] Bognitzki M, Frese T, Steinhart M, Greiner A, Wendorff JH, Schaper A, Hellwig M, Polym. Eng. Sci., 41(6), 982 (2001)

[14] Yang QB, Li ZY, Hong YL, Zhao YY, Qiu SL, Wang C, Wei Y, J. Polym. Sci. B: Polym. Phys., 42(20), 3721 (2004)

[15] Hong JS, Park SR, Lyu MY, Polym.(Korea), 35(1), 1 (2011)

[16] George H. Scherr “Silver alginate foam compositions” U. S. PAT.6,696,077 (2004)

[17] Fong H, Chun I, Reneker DH, Polymer, 40(16), 4585 (1999)

[18] Zong XH, Kim K, Fang DF, Ran SF, Hsiao BS, Chu B, Polymer, 43(16), 4403 (2002)