하수슬러지에서 수소생산 균주의 분리 및 배양조건

우대식; 주현; 이재화; Dae-Sik Woo; Hyun Joo; Jae-Hwa Lee

Applied Chemistry for Engineering, Vol.24, No.4, 407-411, August, 2013

Export Citation

하수슬러지에서 수소생산 균주의 분리 및 배양조건

Isolation and Culture Conditions of Hydrogen-producing Bacterium from Sewage Sludge

우대식, 주현¹, 이재화 ^†

신라대학교 의생명과학대학 생명공학과
¹인제대학교 생리학교실

Dae-Sik Woo, Hyun Joo¹, and Jae-Hwa Lee^†

Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University, Busan 617-736, Korea
¹Department of Physiology, College of Medicine, Inje University, Busan 614-735, Korea

E-mail:

초록

부산시 남부하수처리장 혐기성 하수처리 공정 중 채취한 슬러지에서 수소생산 균주 AS12를 분리하였다. 분리된 균주의 16S rRNA 염기서열을 바탕으로 계통학적 분류 결과 Escherichia coli sp.에 속하는 것으로 동정되었다. 수소생산을 위한 최적 온도와 pH는 각각 35 ℃와 8.0이었다. 배지내의 탄소원과 질소원의 종류 및 농도가 수소생산에 미치는 영향을 알아보았다. 탄소원은 galactose로 10 g/L가 최적이었고, 질소원은 peptone으로 5 g/L가 최적이었다.

Hydrogen producing bacterium, strain AS12 was isolated from the sludge of the anaerobic wastewater treatment process of south sewage treatment plant, Busan city. Phylogenetic analysis based on 16S rRNA sequence studies indicated that AS12 belonged to the genus Escherichia coli sp.. The optimum temperature and pH for hydrogen production were 35 ℃ and 8.0, respectively. The impact of the types and concentrations of carbon and nitrogen sources in the media on hydrogen production was investigated. The optimum carbon and nitrogen concentrations were 10 g/L of galactose and 5 g/L of peptone, respectively.

Keywords:hydrogen production;Escherichia coli;galactose;biological hydrogen production;facultative anaerobe

[References]

Lee CY, Lee SW, J. of KORRA., 19, 49 (2011)
Kim JY, Lee SM, Kim JH, Lee JH, KSBB J., 25, 547 (2010)
Kim KH, Choi YJ, Kim EY, KSBB J., 23, 54 (2008)
Kim MS, Yoon YS, Sim SJ, Park TH, Lee JK, KHNES., 13, 330 (2002)
Yoon WH, Kim HK, Lee TJ, J. KORRA., 14, 131 (2006)
Jo YH, Jo BH, Cha HJ, J. KORRA., 19, 57 (2011)
Kim MS, Oh YK, J. KSEE., 15, 118 (2006)
Jeon SJ, Lee ES, Kor. J. Microbiol. Biotechnol., 38, 399 (2010)
Joo OS, Korean Chem. Eng. Res., 49(6), 688 (2011)
Lee HS, Lee TJ, J. KORRA., 20, 41 (2012)
Kim MS, Baek JS, KSBB J., 20, 393 (2005)
Shin JH, Park TH, Korean Chem. Eng. Res., 44(1), 16 (2006)
Oh YK, Kim MS, KHNES., 19, 41 (2008)
Hwang MH, Jang NJ, Hyun SH, Hong SG, Kim IS, J. KSEE., 24, 1939 (2002)
Choi JY, Jho YC, Ahn IS, Appl. Chem. Eng., 20, 449 (2009)
Penfold DW, Forster CF, Macaskie LE, Enzyme Microb. Technol., 33(2-3), 185 (2003)
Wang J, Wan W, Int. J. Hydrogen Energy., 34, 799 (2009)
Kim CG, Shin HG, J. KORRA., 19, 22 (2011)
Ghosh D, Hallenbeck PC, Int. J. Hydrogen Energy., 34, 7979 (2009)
Alvarado-Cuevas ZD, Ordonez Acevedo LG, Ornelas Salas JT, Leon-Rodriguez AD, N. Biotechnol., In press (2013)
Sa YS, Kim YH, KSBB J., 26, 189 (2011)
Axley MJ, Grahame DA, Stadtman TC, J. Biol. Chem., 265, 18213 (1990)

[Related Articles]

[1] Lee CY, Lee SW, J. of KORRA., 19, 49 (2011)

[2] Kim JY, Lee SM, Kim JH, Lee JH, KSBB J., 25, 547 (2010)

[3] Kim KH, Choi YJ, Kim EY, KSBB J., 23, 54 (2008)

[4] Kim MS, Yoon YS, Sim SJ, Park TH, Lee JK, KHNES., 13, 330 (2002)

[5] Yoon WH, Kim HK, Lee TJ, J. KORRA., 14, 131 (2006)

[6] Jo YH, Jo BH, Cha HJ, J. KORRA., 19, 57 (2011)

[7] Kim MS, Oh YK, J. KSEE., 15, 118 (2006)

[8] Jeon SJ, Lee ES, Kor. J. Microbiol. Biotechnol., 38, 399 (2010)

[9] Joo OS, Korean Chem. Eng. Res., 49(6), 688 (2011)

[10] Lee HS, Lee TJ, J. KORRA., 20, 41 (2012)

[11] Kim MS, Baek JS, KSBB J., 20, 393 (2005)

[12] Shin JH, Park TH, Korean Chem. Eng. Res., 44(1), 16 (2006)

[13] Oh YK, Kim MS, KHNES., 19, 41 (2008)

[14] Hwang MH, Jang NJ, Hyun SH, Hong SG, Kim IS, J. KSEE., 24, 1939 (2002)

[15] Choi JY, Jho YC, Ahn IS, Appl. Chem. Eng., 20, 449 (2009)

[16] Penfold DW, Forster CF, Macaskie LE, Enzyme Microb. Technol., 33(2-3), 185 (2003)

[17] Wang J, Wan W, Int. J. Hydrogen Energy., 34, 799 (2009)

[18] Kim CG, Shin HG, J. KORRA., 19, 22 (2011)

[19] Ghosh D, Hallenbeck PC, Int. J. Hydrogen Energy., 34, 7979 (2009)

[20] Alvarado-Cuevas ZD, Ordonez Acevedo LG, Ornelas Salas JT, Leon-Rodriguez AD, N. Biotechnol., In press (2013)

[21] Sa YS, Kim YH, KSBB J., 26, 189 (2011)

[22] Axley MJ, Grahame DA, Stadtman TC, J. Biol. Chem., 265, 18213 (1990)