Antimicrobial and anticoagulation activity of silver nanoparticles synthesized from the culture supernatant of Pseudomonas aeruginosa

Muniyandi Jeyaraj; Senthilkumar Varadan; Kevin John Pulikotil Anthony; Muralidharan Murugan; Ashok Raja; Sangiliyandi Gurunathan

Journal of Industrial and Engineering Chemistry, Vol.19, No.4, 1299-1303, July, 2013

DOI10.1016/j.jiec.2012.12.031 Export Citation

Antimicrobial and anticoagulation activity of silver nanoparticles synthesized from the culture supernatant of Pseudomonas aeruginosa

Muniyandi Jeyaraj^†, Senthilkumar Varadan, Kevin John Pulikotil Anthony, Muralidharan Murugan, Ashok Raja, and Sangiliyandi Gurunathan^{1, †}

GS Centre for Life Sciences, Gandhinagar, Sundarapuram, Coimbatore, India
¹Department of Animal Biotechnology, Konkuk University, Seoul 143-701, South Korea

E-mail:,

Here, we describe biosynthesis of silver nanoparticles by reduction of aqueous Ag+ ions with the culture supernatant of Pseudomonas aeruginosa. The morphological, physiological, biochemical and molecular level identification of the strain GS1 resembles P. aeruginosa. The nanoparticles synthesized by P. aeruginosa were characterized by UV-vis spectroscopy, dynamic light scattering (DLS) and scanning electron microscopy (SEM). The size-distribution of nanoparticles was determined using a particle-size analyzer and the average particle-size was found to be 80 nm. The biological activities of the synthesized silver nanoparticles like antimicrobial activity were confirmed against Escherichia coli and Staphylococcus aureus and it have stable anti-coagulant effect.

Keywords:Silver nanoparticles;P. aeruginosa;UV-vis spectroscopy;Dynamic light scattering;Scanning electron microscopy (SEM)

[References]

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Ahamed M, Alsalhi MS, Siddiqui MK, Clinica Chimica Acta., 411, 1841 (2010)
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Shahverdi AR, Fakhimi A, Pharm D, Shaverdi HR, Minaian S, Nanomedicine., 3, 168 (2007)
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Yamanaka M, Hara K, Kudo J, Applied and Environment Microbiology., 71, 7589 (2005)
Bragg PD, Rainnie DJ, Journal of Microbiology., 20, 883 (1974)
McDonnell G, Russell A, Clinical Microbiology Reviews., 12, 147 (1999)
Sondi I, Salopek-Sondi B, J. Colloid Interface Sci., 275(1), 177 (2004)
Kemp MM, Kumar A, Mousa S, Park TJ, Ajayan P, Kubotera N, Mousa SA, Linhardt RJ, Biomacromolecules, 10(3), 589 (2009)
Shrivastava S, Bera T, Sunil Singh K, Singh G, Ramachandrarao P, Dash D, ACS Nano., 3, 1357 (2009)

[Referenced By]

[1] Whitesides GM, Nature Biotechnology., 21, 1161 (2003)

[2] Fortin D, Beveridge TJ, Mechanistic routes to biomineral surface development, in: E. Ba¨uerlein (Ed.), Biomineralization: From Biology to Biotechnology and Medical Application, Wiley-VCH, Verlag, Germany, 7 (2000)

[3] Kowshik M, Ashtaputre S, Kharrazi S, Vogel W, Urban J, Kulkarni SK, Paknikar KM, Nanotechnology., 14, 95 (2003)

[4] Kumar SA, Abyaneh MK, Gosavi SW, Kulkarni SK, Pasricha R, Ahmad A, Khan MI, Biotechnol. Lett., 29(3), 439 (2007)

[5] Kalimuthu K, Babu RS, Venkataraman D, Bilal M, Gurunathan S, Colloids and Surfaces B., 65, 150 (2008)

[6] Bhainsa KC, D’Souza SF, Colloids and Surfaces B., 47, 160 (2006)

[7] Shahverdi A, Minaeian S, Shahverdi HR, Jamalifar H, Nohi AA, Process Biochemistry., 42, 919 (2007)

[8] Vigneshwaran N, Ashtaputre NM, Varadarajan PV, Nachane RP, Paralikar KM, Balasubramanya RH, Materials Letters., 66, 1413 (2007)

[9] Gade A, Ingle A, Bawaskar M, Rai M, Journal of Nanoparticle Research., 11, 2079 (2009)

[10] Haefeli C, Franklin C, Hardy K, Journal of Bacteriology., 158, 389 (1984)

[11] Sosa IO, Noguez C, Barrera RG, J. Phys. Chem. B, 107(26), 6269 (2003)

[12] Ahmad A, Mukherjee P, Senapati S, Mandal D, Khan MI, Kumar R, Sastry M, Colloids Surfaces B., 28, 313 (2003)

[13] Ahamed M, Alsalhi MS, Siddiqui MK, Clinica Chimica Acta., 411, 1841 (2010)

[14] Chaloupka K, Malam Y, Seifalian AM, Trends in Biotechnology., 28, 580 (2010)

[15] Pal S, Tak Y, Song J, Applied and Environment Microbiology., 73, 1712 (2007)

[16] Sondi I, Salopek-Sondi B, J. Colloid Interface Sci., 275(1), 177 (2004)

[17] Klaus T, Joerger R, Olsson E, Granqvist CG, Proceedings of the National Academy of Sciences of the United States of America., 96, 13611 (1999)

[18] Cho KH, Park JE, Osaka T, Park SG, Electrochim. Acta, 51(5), 956 (2005)

[19] Mukherjee P, Ahmad A, Mandal D, Senapati S, Sainkar SR, Khan MI, Parischa R, Ajayakumar PV, Alam M, Kumar R, Sastry M, Nano Letters., 1, 515 (2001)

[20] Duran, Marcato PD, Alves OL, Souza G, Souza GI, Esposito E, Journal of Biotechnology., 3, 8 (2005)

[21] Alt V, Bechert T, Steinrucke P, Wagener M, Seidel P, Dingeldein E, Biomaterials., 25, 4383 (2004)

[22] Arora S, Jain J, Rajwade JM, Paknikar KM, Toxicology Letters., 179, 93 (2008)

[23] Nadworny PL, Wang J, Tredget EE, Burrell RE, Nanotechnology, Biology, and Medicine., 3, 241 (2008)

[24] Kim K, Sung W, Suh B, Moon S, Choi J, Kim J, Lee D, Biometals., 22, 235 (2009)

[25] Rogers JV, Parkinson CV, Choi YW, Speshock JL, Hussain SM, Research Letters., 3, 129 (2008)

[26] Holt K, Bard A, Biochemistry., 44, 13214 (2005)

[27] Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramirez JT, Yacaman MJ, Journal of Nanotechnology., 16, 2346 (2005)

[28] Kurihara K, Rockstuhl C, Nakano T, Arai T, Tominaga J, Nanotechnology., 16, 1565 (2005)

[29] Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST, Bergey’s Manual of Determinative Bacteriology, 9th Ed., Williamsons and Wilkins, Baltimore (1994)

[30] Gurunathan S, Kalishwaralal K, Vaidyanathan R, Venkataraman D, Pandian SRK, Muniyandi J, Hariharan N, Eom SH, Colloids Surfaces B., 74, 328 (2009)

[31] Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins, Nucleic Acids Research., 25, 4876 (1997)

[32] Maidak LB, Cole JR, Lilburn TG, Parker CT, Saxman PR, Farris RJ, Garrity GM, Olsen GJ, Schmidt TM, Tiedje JM, Nucleic Acids Research., 29, 173 (2001)

[33] Kalishwaralal K, Deepak V, Ramkumarpandian S, Nellaiah H, Sangiliyandi G, Materials Letters., 62, 4411 (2008)

[34] Naik RR, Stringer SJ, Agarwal G, Jones SE, Stone MO, Nat. Mater., 1(3), 169 (2002)

[35] Sastry M, Mayyaa KS, Bandyopadhyay K, Colloids Surfaces A., 127, 221 (1997)

[36] Sastry M, Patil V, Sainkar SR, J. Phys. Chem. B, 102(8), 1404 (1998)

[37] Pandian SRK, Deepak V, Kalishwaralal K, Viswanathan P, Gurunathan S, Brazilian Journal of Microbiology, 41, 805 (2010)

[38] Ahmad A, Mukherjee P, Senapati S, Manda ID, Khan MI, Kumar R, Colloids and Surfaces B., 28, 313 (2003)

[39] Shahverdi AR, Fakhimi A, Pharm D, Shaverdi HR, Minaian S, Nanomedicine., 3, 168 (2007)

[40] Feng QL, Wu J, Chen GQ, Cui FZ, Kim TM, Kim JO, Journal of Biomedical Materials Research., 52, 662 (2000)

[41] Yamanaka M, Hara K, Kudo J, Applied and Environment Microbiology., 71, 7589 (2005)

[42] Bragg PD, Rainnie DJ, Journal of Microbiology., 20, 883 (1974)

[43] McDonnell G, Russell A, Clinical Microbiology Reviews., 12, 147 (1999)

[44] Sondi I, Salopek-Sondi B, J. Colloid Interface Sci., 275(1), 177 (2004)

[45] Kemp MM, Kumar A, Mousa S, Park TJ, Ajayan P, Kubotera N, Mousa SA, Linhardt RJ, Biomacromolecules, 10(3), 589 (2009)

[46] Shrivastava S, Bera T, Sunil Singh K, Singh G, Ramachandrarao P, Dash D, ACS Nano., 3, 1357 (2009)