This work involved the use of a hydrocyclone to separate yeast from alcoholic fermentation suspensions. The performance of the hydrocyclone was quantified using empirical models that describe the capacity, total separation efficiency, flow ratio, and reduced separation efficiency as a function of the concentration of the suspension and the operating pressure drop. The results showed capacities of 0.1283 to 0.1820m3/h, total separation efficiencies of 81.96% to 92.19%, reduced separation efficiencies of 0.63% to 58.44%, and flow ratios ranging from 81.05% to 84.85%.