In this study, a simple, rapid, and efficient purification method of five major flavonoids from crude Flaveria bidentis extracts was established. Five flavonoids components were initially obtained by ethanol-water extraction of Flaveria bidentis (L.) Kuntze, followed by using D4020 resin-based column chromatography and semi-preparative high performance liquid chromatography. 9.3mg hyperoside, 6.5mg patuletin-3-O-glucoside, 1.7mg isorhamnetin 3-sulphate, 8.4mg astragalin, and 4.9mg 6-methoxykaempferol-3-O-galactoside at high purity of over 94% were obtained from 580mg Flaveria bidentis extracts. This method is more efficient than HSCCC comparing with the results of these two methods regarding analysts' purity, recovery, and running time.