비타민나무 잎 추출물의 피부 흡수 증진을 위한 나노에멀젼 연구

채교영; 권순식; 박수남; Kyo Young Chae; Soon Sik Kwon; Soo Nam Park

Applied Chemistry for Engineering, Vol.24, No.3, 260-265, June, 2013

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비타민나무 잎 추출물의 피부 흡수 증진을 위한 나노에멀젼 연구

A Study on Nano-emulsion for Enhanced Transdermal Delivery of Hippophae rhamnoides Leaf Extract

채교영, 권순식, 박수남 ^†

서울과학기술대학교 정밀화학과 나노바이오화장품연구실 화장품종합기술연구소

Kyo Young Chae, Soon Sik Kwon, and Soo Nam Park^†

Department of Fine Chemistry, Nanobiocosmetic Laboratory and Cosmetic R&D Center, Seoul National University of Science and Technology, Seoul 139-743, Korea

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초록

본 연구에서는 비타민나무 잎 추출물의 에틸아세테이트 분획 0.01, 0.03, 0.05, 0.10% 함유한 나노에멀젼을 제조하였고, 5주 동안 이들 나노에멀젼의 입자 크기, 입도분포 및 피부 투과능을 평가하였다. 나노에멀젼은 균질기(homogenizer) 처리 후 고압유화기(micro-fluidizer)를 이용하여 제조하였다. 비타민나무 잎 추출물의 에틸아세테이트 분획을 함유한 나노에멀젼은 단분산 형태를 나타내었다. 5주 동안 실험에서, 0.03% 에틸아세테이트 분획을 함유한 나노에멀젼이 가장 안정하였다. 0.03% 에틸아세테이트 분획 함유 나노에멀젼의 in vitro 피부 투과 실험을 Franz diffusion cell을 이용하여 수행하였다. Oil-in-water (O/W) 에멀젼과 비교할 때 나노에멀젼이 피부 흡수가 더 잘되는 것으로 나타났다. 이러한 결과들은 비타민나무 잎 추출물을 함유한 나노에멀젼이 O/W 에멀젼보다 안정성과 피부 투과능이 우수함을 나타낸다.

In this study, nano-emulsions containing 0.01, 0.03, 0.05, and 0.10% ethyl acetate fraction of Hippophae rhamnoides (H.rhamnoides) leaf extracts were prepared. The particle size, particle size distribution and skin permeability of the nano-emulsions were evaluated for five weeks. Nano-emulsion was prepared by the sequential use of homogenizer and microfluidizer. Nano-emulsion containing the ethyl acetate fraction exhibited a monodispersed form. Nano-emulsion containing 0.03% ethyl acetate fraction was the most stable for five weeks. The in vitro skin permeation study of nano-emulsion containing 0.03% ethyl acetate fraction was carried out using Franz diffusion cell. The nano-emulsion showed a better skin permeability than that of O/W emulsion. These results indicate that the nano-emulsion containing the ethyl acetate fraction of H. rhamnoides leaf extract showed a remarkable stability and skin permeability than that of O/W emulsion.

Keywords:Hippophae rhamnoides leaf;nano-emulsion;transdermal delivery;Franz diffusion cell

[References]

Kyong KY, Lee CK, J. Soc. Cosmet. Scientists Korea., 32, 214 (2006)
Cho WG, AAPG Bull., 37, 1 (2011)
Tadros T, Izduierdo P, Esquena J, Solans C, Adv. Colloid Interface Sci., 180, 303 (2004)
Forgiarini A, Esquena J, Gonzalez J, Solans C, Prog.Colloid Polym. Sci., 115, 36 (2000)
Gutierrez JM, Gonzalez C, Maestro A, Sole I, Pey CM, Nolla J, Curr. Opin. Colloid Interface Sci., 13, 245 (2008)
Won BR, Kang MK, Park SN, J. Soc. Cosmet. Scientists Korea., 35, 79 (2009)
Jeong HS, Yoo JY, Lee HS, Seo JH, Ko WB, KIC News., 12, 1 (2009)
Kim KM, Park MH, Kim KH, Im SH, Park YH, Kim YN, J. Appl. Boil. Chem., 52, 58 (2009)
Chauhan AS, Negi PS, Fitoterapia, RS
Padwad Y, Ganju L, Jain M, Int Immunopharmacol., 6, 46 (2006)
Tiffany TYG, Stefan C, Arnie H, J. Food. Sci., 70, 514 (2005)
Kim JS, Yu CY, Kim MJ, J. Plant Biotechnol., 37, 47 (2010)
Kim JE, Chae KY, Park, SN, J. Soc. Cosmet. Scientists Korea., 37, 265 (2011)
Baik SS, J. Soc. Cosmet. Scientists Korea., 19, 127 (1993)
Cho WG, J. Kor. Oil Chem. Soc., 69, 410 (2007)
Lim MS, Park MA, Park SN, Appl. Chem. Eng., 23(2), 222 (2012)
Godin B, Touitou E, Advanced Drug Delivery Reviews., 59, 1152 (2007)
Gupta A, Kumar R, Pal K, Banerjee PK, Sawhney RC, The Int. J. of Low. Extremity Wounds., 4, 88 (2005)
Zu Y, Li C, Fu Y, Zhao C, J. Pharmaceut. Biomed. Anal., 41, 714 (2006)
Cho WG, J. Korean Oil Chemists Soc., 37, 97 (2011)

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[2] Cho WG, AAPG Bull., 37, 1 (2011)

[3] Tadros T, Izduierdo P, Esquena J, Solans C, Adv. Colloid Interface Sci., 180, 303 (2004)

[4] Forgiarini A, Esquena J, Gonzalez J, Solans C, Prog.Colloid Polym. Sci., 115, 36 (2000)

[5] Gutierrez JM, Gonzalez C, Maestro A, Sole I, Pey CM, Nolla J, Curr. Opin. Colloid Interface Sci., 13, 245 (2008)

[6] Won BR, Kang MK, Park SN, J. Soc. Cosmet. Scientists Korea., 35, 79 (2009)

[7] Jeong HS, Yoo JY, Lee HS, Seo JH, Ko WB, KIC News., 12, 1 (2009)

[8] Kim KM, Park MH, Kim KH, Im SH, Park YH, Kim YN, J. Appl. Boil. Chem., 52, 58 (2009)

[9] Chauhan AS, Negi PS, Fitoterapia, RS

[10] Padwad Y, Ganju L, Jain M, Int Immunopharmacol., 6, 46 (2006)

[11] Tiffany TYG, Stefan C, Arnie H, J. Food. Sci., 70, 514 (2005)

[12] Kim JS, Yu CY, Kim MJ, J. Plant Biotechnol., 37, 47 (2010)

[13] Kim JE, Chae KY, Park, SN, J. Soc. Cosmet. Scientists Korea., 37, 265 (2011)

[14] Baik SS, J. Soc. Cosmet. Scientists Korea., 19, 127 (1993)

[15] Cho WG, J. Kor. Oil Chem. Soc., 69, 410 (2007)

[16] Lim MS, Park MA, Park SN, Appl. Chem. Eng., 23(2), 222 (2012)

[17] Godin B, Touitou E, Advanced Drug Delivery Reviews., 59, 1152 (2007)

[18] Gupta A, Kumar R, Pal K, Banerjee PK, Sawhney RC, The Int. J. of Low. Extremity Wounds., 4, 88 (2005)

[19] Zu Y, Li C, Fu Y, Zhao C, J. Pharmaceut. Biomed. Anal., 41, 714 (2006)

[20] Cho WG, J. Korean Oil Chemists Soc., 37, 97 (2011)