pH 감응성 P(MAA-co-PEGMA) 수화젤이 알부민의 방출과 안정성에 미치는 영향

양주승; 김범상; Juseung Yang; Bumsang Kim

Polymer(Korea), Vol.37, No.3, 262-268, May, 2013

Export Citation

pH 감응성 P(MAA-co-PEGMA) 수화젤이 알부민의 방출과 안정성에 미치는 영향

Effect of pH-Sensitive P(MAA-co-PEGMA) Hydrogels on Release and Stability of Albumin

양주승, 김범상 ^†

홍익대학교 화학공학과

Juseung Yang, and Bumsang Kim^†

Department of Chemical Engineering, Hongik University, 94 Wausan-ro, Mapo-gu, Seoul 121-791, Korea

E-mail:

초록

본 연구에서는 pH 감응성 수화젤 입자를 이용하여 외부환경에 불안정한 활성물질을 화장품 제형 내에서는 안정하게 보존하고, 피부에 도포 시 빠른 방출로 피부에 흡수될 수 있는 지능형 전달시스템의 구현 가능성을 확인하기 위하여, 분산광중합을 이용하여 pH 감응성을 가지는 P(MAA-co-PEGMA) 수화젤 입자를 평균 크기 약 2 μm의 구형 입자로 합성하였다. 합성된 P(MAA-co-PEGMA) 수화젤 입자는 수화젤의 MAA에 존재하는 카르복시기의 이온화에 의하여 pH 5를 전후로 한 급격한 팽윤비의 변화를 보여주었다. pH에 따른 수화젤 내부에 탑재된 알부민의 방출 및 피부투과 실험결과, P(MAA-co-PEGMA) 수화젤 입자는 pH 4.0에서는 소량의 알부민이 방출되어 피부투과가 거의 일어나지 않은 반면, pH 6.0에서는 초기부터 다량의 알부민이 방출되어 상대적으로 높은 피부투과율을 나타내었다. 펩신을 이용한 알부민의 안정성 실험결과, P(MAA-co-PEGMA) 수화젤은 내부에 탑재된 알부민을 외부환경으로부터 보호하여 알부민의 안정성을 유지시켜 주었다.

pH-sensitive P(MAA-co-PEGMA) hydrogel particles were prepared and their feasibility as smart delivery carriers for cosmetic ingredients was evaluated. P(MAA-co-PEGMA) hydrogel particles having an average size of approx. 2 μm were synthesized via dispersion photopolymerization. There was a drastic change in the swelling ratio of P(MAAco-PEGMA) particles at a pH of around 5 due to the ionization of MAA in the hydrogel and as the amount of MAA in the hydrogel increased, the swelling ratio increased at a pH above 5. The P(MAA-co-PEGMA) hydrogel particles showed a pH-sensitive release behavior. Thus, at pH 4 almost none of the albumin permeated through the skin while at pH 6 relatively high skin permeability was obtained. The albumin loaded in the P(MAA-co-PEGMA) hydrogel particles was hardly degraded in the presence of pepsin and its stability was maintained.

Keywords:pH-sensitive;hydrogel particles;albumin stability;smart delivery system;skin permeability.

[References]

Peppas NA, Bures P, Leobandung W, Ichikawa H, Eur. J.Pharm. Biopharm., 50, 27 (2000)
Chung BG, Lee KH, Khademhosseini A, Lee SH, Lab Chip., 12, 45 (2012)
Gonzalez-Chomon C, Concheiro A, Alvarez-Lorenzo C, Materials., 4, 1927 (2011)
Cabral J, Moratti SC, Future Med. Chem., 3, 1877 (2011)
Jabbari E, Curr. Opin. Biotechnol., 22, 655 (2011)
Jeong B, Kim SW, Bae YH, Adv. Drug Deliv. Rev., 54, 37 (2002)
Tomatsu I, Peng K, Kros A, Adv. Drug Deliv. Rev., 63, 1257 (2011)
Li Z, Guan J, Expert Opin. Drug Deliv., 8, 991 (2011)
Zha L, Banik B, Alexis F, Soft Matter., 7, 5908 (2011)
Bajpai AK, Bajpai J, Saini R, Gupta R, Polym. Rev., 51, 53 (2011)
Foss AC, Goto T, Morishita M, Peppas NA, Eur. J.Pharm. Biopharm., 57, 163 (2004)
Mocanu G, Mihai D, Dulong V, Picton L, Le Cerf D, Carbohydr. Polym., 87, 1440 (2012)
Kim B, Kim SH, Ryoo W, J. Biomater. Sci.-Polym. Ed., 20, 427 (2009)
Lee E, Kim B, Polym. Bull., 67(1), 67 (2011)
Wood KM, Stone GM, Peppas NA, Acta Biomater., 6, 48 (2010)
Zhang SG, Nat. Mater., 3(1), 7 (2004)
Revzin A, Russell RJ, Yadavalli VK, Koh WG, Deister C, Hile DD, Mellott MB, Pishko MV, Langmuir, 17(18), 5440 (2001)
Zhu J, Marchant RE, Expert Rev. Med. Devices., 8, 607 (2011)
Kligman AM, Papa CM, J. Soc. Cosmetic Chemists., 16, 557 (1965)
Peters T, All about Albumin: Biochemistry, Genetics, and Medical Applications, Academic Press, New York (1996)
Jiang R, Hayden CGJ, Prankerd RJ, Roberts MS, Benson HAE, J. Chromatogr. B., 682, 137 (1996)
Hasse S, Kothari S, Rokos H, Kauser S, Schurer NY, Schallreuter KU, Exp. Dermatol., 14, 182 (2005)
Kerr A, Ferguson J, Photodermatol. Photoimmunol.Photomed., 26, 56 (2010)
Sharma V, Jaishankar A, Wang YC, McKinley GH, Soft Matter., 7, 5150 (2011)

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[2] Chung BG, Lee KH, Khademhosseini A, Lee SH, Lab Chip., 12, 45 (2012)

[3] Gonzalez-Chomon C, Concheiro A, Alvarez-Lorenzo C, Materials., 4, 1927 (2011)

[4] Cabral J, Moratti SC, Future Med. Chem., 3, 1877 (2011)

[5] Jabbari E, Curr. Opin. Biotechnol., 22, 655 (2011)

[6] Jeong B, Kim SW, Bae YH, Adv. Drug Deliv. Rev., 54, 37 (2002)

[7] Tomatsu I, Peng K, Kros A, Adv. Drug Deliv. Rev., 63, 1257 (2011)

[8] Li Z, Guan J, Expert Opin. Drug Deliv., 8, 991 (2011)

[9] Zha L, Banik B, Alexis F, Soft Matter., 7, 5908 (2011)

[10] Bajpai AK, Bajpai J, Saini R, Gupta R, Polym. Rev., 51, 53 (2011)

[11] Foss AC, Goto T, Morishita M, Peppas NA, Eur. J.Pharm. Biopharm., 57, 163 (2004)

[12] Mocanu G, Mihai D, Dulong V, Picton L, Le Cerf D, Carbohydr. Polym., 87, 1440 (2012)

[13] Kim B, Kim SH, Ryoo W, J. Biomater. Sci.-Polym. Ed., 20, 427 (2009)

[14] Lee E, Kim B, Polym. Bull., 67(1), 67 (2011)

[15] Wood KM, Stone GM, Peppas NA, Acta Biomater., 6, 48 (2010)

[16] Zhang SG, Nat. Mater., 3(1), 7 (2004)

[17] Revzin A, Russell RJ, Yadavalli VK, Koh WG, Deister C, Hile DD, Mellott MB, Pishko MV, Langmuir, 17(18), 5440 (2001)

[18] Zhu J, Marchant RE, Expert Rev. Med. Devices., 8, 607 (2011)

[19] Kligman AM, Papa CM, J. Soc. Cosmetic Chemists., 16, 557 (1965)

[20] Peters T, All about Albumin: Biochemistry, Genetics, and Medical Applications, Academic Press, New York (1996)

[21] Jiang R, Hayden CGJ, Prankerd RJ, Roberts MS, Benson HAE, J. Chromatogr. B., 682, 137 (1996)

[22] Hasse S, Kothari S, Rokos H, Kauser S, Schurer NY, Schallreuter KU, Exp. Dermatol., 14, 182 (2005)

[23] Kerr A, Ferguson J, Photodermatol. Photoimmunol.Photomed., 26, 56 (2010)

[24] Sharma V, Jaishankar A, Wang YC, McKinley GH, Soft Matter., 7, 5150 (2011)