Utilization of cane molasses for docosahexaenoic acid production by Schizochytrium sp. CCTCC M209059

Lu-Jing Ren; Juan Li; Yuan-Wei Hu; Xiao-Jun Ji; He Huang

Korean Journal of Chemical Engineering, Vol.30, No.4, 787-789, April, 2013

DOI10.1007/s11814-013-0020-0 Export Citation

Utilization of cane molasses for docosahexaenoic acid production by Schizochytrium sp. CCTCC M209059

Lu-Jing Ren, Juan Li, Yuan-Wei Hu, Xiao-Jun Ji, and He Huang^†

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing 210009, P. R. China

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Cane molasses (CM), an agro-industrial by-product, was first examined for docosahexaenoic acid (DHA) production by Schizochytrium sp. Cell dry weight as 21.94 g/L at treated CM cultivation was similar to that at pure glucose (26.7 g/L) cultivation. Batch fermentation at different initial CM concentration showed that DHA percentage could reach 47.51% at 10 g/L CM but only 37.90% at 70 g/L CM. By analyzing the fermentation process, monosodium glutamate might be a positive agent for effective DHA production. Finally, monosodium glutamate and malic acid were introduced to the fed-batch fermentation for effective DHA production.

Keywords:Docosahexaenoic Acid;Schizochytrium sp.;Cane Molasses;Lipid;Monosodium Glutamate

[References]

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[1] Agostoni C, Riva E, Trojan S, Bellu R, Giovannini M, Lancet., 346, 638 (1995)

[2] Sijtsma L, de Swaaf ME, Appl. Microbiol. Biotechnol., 64(2), 146 (2004)

[3] Ganuza E, Anderson AJ, Ratledge C, Biotechnol. Lett., 30(9), 1559 (2008)

[4] Liang YN, Sarkany N, Cui Y, Blackburn JW, Bioresour. Technol., 101(17), 6745 (2010)

[5] Unagal P, Assantachai C, Phadungruengluij S, Suphantharika M, Tanticharoen M, Verduyn C, Bioresour. Technol., 98(2), 281 (2007)

[6] Liang Y, Sarkany N, Cui Y, Yesuf J, Trushenski J, Blackburn JW, Bioresour. Technol., 101, 3023 (2010)

[7] Liu YP, Zheng P, Sun ZH, Ni Y, Dong JJ, Zhu LL, Bioresour. Technol., 99, 1736 (2010)

[8] El-Enshasy HA, Mohamed NA, Farid MA, El-Diwany AI, Bioresour. Technol., 99(10), 4263 (2008)

[9] Li N, Deng ZN, Qin YL, Chen CL, Liang ZQ, Food Technol. Biotechnol., 46, 73 (2008)

[10] Ren LJ, Huang H, Xiao AH, Lian M, Ji XJ, Bioproc. Biosyst. Eng., 32, 837 (2009)

[11] Ye Q, Li XM, Yan M, Cao H, Xu L, Zhang YY, Chen Y, Xiong J, Ouyang PK, Ying HJ, Appl. Microbiol. Biotechnol., 87(2), 517 (2010)

[12] Miller GL, Anal. Chem., 31, 426 (1959)

[13] Ren LJ, Ji XJ, Huang H, Qu LA, Feng Y, Tong QQ, Ouyang PK, Appl. Microbiol. Biotechnol., 87(5), 1649 (2010)

[14] Yu LJ, Qin WM, Lan WZ, Zhou PP, Zhu M, Bioresour. Technol., 88(3), 265 (2003)

[15] Ratledge C, Biochimie., 86, 807 (2004)

[16] Edmund M, Wise J, Eric GB, Proc. Natl. Acad Sci., 52(5), 1255 (1964)

[17] Wedding RT, Plant Physiol., 90, 367 (1989)

[18] Lan WZ, Qin WM, Yu LJ, Lett. Appl. Microbiol., 35, 357 (2002)