In vitro evaluation of UHMWPE/zirconia composite using human peripheral blood mononuclear cells

Ki-Dong Yoo; Gee-Hee Kim; Dong Il Noh; Ju Woong Jang; Young Bok Shim; Heung Jae Chun

Macromolecular Research, Vol.21, No.1, 108-113, January, 2013

DOI10.1007/s13233-013-1055-0 Export Citation

In vitro evaluation of UHMWPE/zirconia composite using human peripheral blood mononuclear cells

Ki-Dong Yoo^{1, 2}, Gee-Hee Kim^{1, 2}, Dong Il Noh³, Ju Woong Jang³, Young Bok Shim³, and Heung Jae Chun^{4, 2, †}

¹Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Gyeonggi, 442-723, Korea
²Institute of Cell & Tissue Engineering, College of Medicine, The Catholic University of Korea, Seoul, 137-701, Korea
³Research Institute of Biomedical Engineering, Korea Bone Bank Co. Ltd., Seoul, 153-782, Korea
⁴Department of Biomedical Sciences, College of Medicine, The Catholic University of Korea, Seoul, 137-701, Korea

E-mail:

The objective of the present study was to investigate in vitro biocompatibility of ultra high molecular weight polyethylene (UHMWPE)/zirconia-polymerized composite (PC) using human peripheral blood mononuclear cells (PBMCs). This study was undertaken to compare the levels of free radical generations for neat UHMWPE and PC through irradiation sterilization, and their response to PBMCs viability. Electron spin resonance (ESR) studies showed that γ-ray irradiation of the samples generated free radicals; the extent was inversely related to zirconia content, and the free radicals strongly influenced cell viability. DNA fragmentation and DAPI staining assays revealed that cell death was associated with the induction of apoptosis. Flow cytometry also showed that cell death was largely dependent upon both early and late apoptosis, and, importantly, oxidized PC (ox-PC) exhibited a significantly lower rate for late apoptosis compared to that of oxidized UHMWPE (ox-UHMWPE).

Keywords:ultra high molecular weight polyethylene;zirconia;composite;peripheral blood mononuclear cells;free radicals;cytotoxicity;apoptosis

[References]

Costa L, Luda MP, Trossarelli L, Brach del Prever EM, Crova M, Gallinaro P, Biomaterials., 19, 659 (1998)
Fang L, Leng Y, Gao P, Biomaterials., 26, 3471 (2005)
Goldman M, Lee M, Gronsky R, Pruitt L, J. Biomed.Mater. Res., 37, 43 (1997)
Lee CS, Jho JY, Choi K, Hwang TW, Macromol. Res., 12(1), 141 (2004)
Lee CS, Yoo SH, Jho JY, Choi K, Hwang TW, Macromol. Res., 12(1), 112 (2004)
Medel F, Gomez-Barrena E, Garcia-Alvarez F, Rios R, Gracia-Villa L, Puertolas JA, Biomaterials., 25, 9 (2004)
Reno F, Lombardi F, Cannas M, Biomaterials., 24, 17 (2003)
Reno F, Bracco P, Lombardi F, Boccafoschi F, Costa L, Cannas M, Biomaterials., 25, 6 (2004)
Bosetti M, Zanardi L, Bocco P, Costa L, Cannas M, Biomaterials., 24, 8 (2003)
Rincheval V, Bergeaud M, Mathieu L, leory J, Guillaume A, Mignotte B, Le Floch N, Vayssiere JL, Cell Biol. Toxicol. (2012)
Denecker G, Vercammen D, Declercq W, Vandenabeele P, Cell. Mol. Life Sci., 58, 3 (2001)
Kaufman AM, Alabre CL, Rubash HE, Shanbhag AS, J. Biomed. Mater. Res. A., 84, 2 (2008)
Rosa SML, Nachtigall SMB, Ferreira CA, Macromol. Res., 17(1), 8 (2009)
Klopffer W, in Introduction to Polymer Spectroscopy, Springer-Verlag, New York, 1984, Part D.
Nakamura K, Ogata S, Ikada Y, Biomaterials., 19, 24 (1998)
Slouf M, Synkova H, Baldrian J, Marek A, Kovarova J, Schmidt P, Dorschner H, Stephan M, Gohs U, J. Biomed.Mater. Res. B: Appl. Biomater., 85B, 240 (2008)
Halliwell B, Gutteridge JMC, in Free Radicals in Biology and Medicine, Oxford Univ. Press, Inc., New York, 251 (1999)
Choi J, Park C, Choi JO, Rhim H, Chun HJ, J.Microbiol. Biotechnol., 11, 3 (2001)
Hingorani R, Deng J, Elia J, McIntyre C, Mittar D, in Application Note: Detection of Apoptosis Using the BD Annexin V FITC Assay on the BD FACSVerseTM System, BD Biosciences, San Jose (2011)
darzynkiewicz Z, Juan G, Li X, Gorczyca W, Murakami T, Traganos F, Cytometry., 27, 1 (1997)
Reno F, Sabbatini M, Cannas M, J. Mater. Sci. Mater.Med., 14, 241 (2003)
Franz S, Frey B, Sheriff A, Gaipl US, Beer A, Voll R, Kalden JR, Herrmann M, Cytometry A., 69A, 230 (2006)
Jang HS, Ryu KE, Ahn WS, Chun HJ, Park HD, Park KD, Kim YH, Colloids Surf. B: Biointerfaces., 50, 2 (2006)

[Referenced By]

[1] Costa L, Luda MP, Trossarelli L, Brach del Prever EM, Crova M, Gallinaro P, Biomaterials., 19, 659 (1998)

[2] Fang L, Leng Y, Gao P, Biomaterials., 26, 3471 (2005)

[3] Goldman M, Lee M, Gronsky R, Pruitt L, J. Biomed.Mater. Res., 37, 43 (1997)

[4] Lee CS, Jho JY, Choi K, Hwang TW, Macromol. Res., 12(1), 141 (2004)

[5] Lee CS, Yoo SH, Jho JY, Choi K, Hwang TW, Macromol. Res., 12(1), 112 (2004)

[6] Medel F, Gomez-Barrena E, Garcia-Alvarez F, Rios R, Gracia-Villa L, Puertolas JA, Biomaterials., 25, 9 (2004)

[7] Reno F, Lombardi F, Cannas M, Biomaterials., 24, 17 (2003)

[8] Reno F, Bracco P, Lombardi F, Boccafoschi F, Costa L, Cannas M, Biomaterials., 25, 6 (2004)

[9] Bosetti M, Zanardi L, Bocco P, Costa L, Cannas M, Biomaterials., 24, 8 (2003)

[10] Rincheval V, Bergeaud M, Mathieu L, leory J, Guillaume A, Mignotte B, Le Floch N, Vayssiere JL, Cell Biol. Toxicol. (2012)

[11] Denecker G, Vercammen D, Declercq W, Vandenabeele P, Cell. Mol. Life Sci., 58, 3 (2001)

[12] Kaufman AM, Alabre CL, Rubash HE, Shanbhag AS, J. Biomed. Mater. Res. A., 84, 2 (2008)

[13] Rosa SML, Nachtigall SMB, Ferreira CA, Macromol. Res., 17(1), 8 (2009)

[14] Klopffer W, in Introduction to Polymer Spectroscopy, Springer-Verlag, New York, 1984, Part D.

[15] Nakamura K, Ogata S, Ikada Y, Biomaterials., 19, 24 (1998)

[16] Slouf M, Synkova H, Baldrian J, Marek A, Kovarova J, Schmidt P, Dorschner H, Stephan M, Gohs U, J. Biomed.Mater. Res. B: Appl. Biomater., 85B, 240 (2008)

[17] Halliwell B, Gutteridge JMC, in Free Radicals in Biology and Medicine, Oxford Univ. Press, Inc., New York, 251 (1999)

[18] Choi J, Park C, Choi JO, Rhim H, Chun HJ, J.Microbiol. Biotechnol., 11, 3 (2001)

[19] Hingorani R, Deng J, Elia J, McIntyre C, Mittar D, in Application Note: Detection of Apoptosis Using the BD Annexin V FITC Assay on the BD FACSVerseTM System, BD Biosciences, San Jose (2011)

[20] darzynkiewicz Z, Juan G, Li X, Gorczyca W, Murakami T, Traganos F, Cytometry., 27, 1 (1997)

[21] Reno F, Sabbatini M, Cannas M, J. Mater. Sci. Mater.Med., 14, 241 (2003)

[22] Franz S, Frey B, Sheriff A, Gaipl US, Beer A, Voll R, Kalden JR, Herrmann M, Cytometry A., 69A, 230 (2006)

[23] Jang HS, Ryu KE, Ahn WS, Chun HJ, Park HD, Park KD, Kim YH, Colloids Surf. B: Biointerfaces., 50, 2 (2006)